TY - JOUR
T1 - The 67 kDa laminin receptor as a primary determinant of anti-allergic effects of O-methylated EGCG
AU - Fujimura, Yoshinori
AU - Umeda, Daisuke
AU - Yano, Satomi
AU - Maeda-Yamamoto, Mari
AU - Yamada, Koji
AU - Tachibana, Hirofumi
N1 - Funding Information:
This work was supported in part by grants from the Bio-oriented Technology Research Advancement Institution to H.T.
PY - 2007/12/7
Y1 - 2007/12/7
N2 - Previously we have reported that the O-methylated derivative of (-)-epigallocatechin-3-O-gallate (EGCG), (-)-epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3"Me), possesses anti-allergic activities such as inhibition of histamine release and suppression of the high-affinity IgE receptor (FcεRI) expression. However, the underlying mechanism is still unclear. Recently we have identified the 67 kDa laminin receptor (67LR) as a cell-surface receptor that can mediate biological activities of EGCG. Here we show that the suppression of myosin II regulatory light chain (MRLC) phosphorylation through the cell-surface binding to the 67 LR contributes to the inhibitory effect of EGCG3"Me on the histamine release from the human basophilic KU812 cells. The 67LR also mediated the EGCG3"Me-induced suppression of FcεRI expression by reducing ERK1/2 phosphorylation. These results suggest that anti-allergic effects of EGCG3"Me may be triggered by the inhibition of MRLC or ERK1/2 phosphorylation mediated through the cell-surface 67LR.
AB - Previously we have reported that the O-methylated derivative of (-)-epigallocatechin-3-O-gallate (EGCG), (-)-epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3"Me), possesses anti-allergic activities such as inhibition of histamine release and suppression of the high-affinity IgE receptor (FcεRI) expression. However, the underlying mechanism is still unclear. Recently we have identified the 67 kDa laminin receptor (67LR) as a cell-surface receptor that can mediate biological activities of EGCG. Here we show that the suppression of myosin II regulatory light chain (MRLC) phosphorylation through the cell-surface binding to the 67 LR contributes to the inhibitory effect of EGCG3"Me on the histamine release from the human basophilic KU812 cells. The 67LR also mediated the EGCG3"Me-induced suppression of FcεRI expression by reducing ERK1/2 phosphorylation. These results suggest that anti-allergic effects of EGCG3"Me may be triggered by the inhibition of MRLC or ERK1/2 phosphorylation mediated through the cell-surface 67LR.
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U2 - 10.1016/j.bbrc.2007.09.095
DO - 10.1016/j.bbrc.2007.09.095
M3 - Article
C2 - 17927962
AN - SCOPUS:35348945881
SN - 0006-291X
VL - 364
SP - 79
EP - 85
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -