The 5′-region of the P450XIA1 (P450scc) gene contains a basal promoter and an adrenal-specific activating domain

Bon chu Chung; Meng Chun Hu; Char Chang Lai; Cheng Hui Lin

doi:10.1016/0006-291X(89)91652-5

The 5′-region of the P450XIA1 (P450scc) gene contains a basal promoter and an adrenal-specific activating domain

Bon chu Chung, Meng Chun Hu, Char Chang Lai, Cheng Hui Lin

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

The first step to the synthesis of all steroids is catalyzed by P450scc. We constructed nine deletion mutants of the 5′-region of the P450scc gene and connected them to a CAT reporter gene to assay transcriptional activity of the P450scc promoter. A short 145 bp fragment stimulated transcriptionaby two fold. This DNA was active in all cells tested irrespective of their tissue origin and steroidogenic activity. DNA at -145 -573 of the upstream region did not increase transcription any further. DNA including 2500 bp of the upstream region stimulated transcription by 10 fold only in adrenal Y-1 cells. Hence in the -145 region contains a low level P450scc promoter and the 2500 bp DNA possesses an adrenal specific enhancing element.

Original language	English
Pages (from-to)	276-281
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	160
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(89)91652-5
Publication status	Published - Apr 14 1989
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(89)91652-5

Cite this

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title = "The 5′-region of the P450XIA1 (P450scc) gene contains a basal promoter and an adrenal-specific activating domain",

abstract = "The first step to the synthesis of all steroids is catalyzed by P450scc. We constructed nine deletion mutants of the 5′-region of the P450scc gene and connected them to a CAT reporter gene to assay transcriptional activity of the P450scc promoter. A short 145 bp fragment stimulated transcriptionaby two fold. This DNA was active in all cells tested irrespective of their tissue origin and steroidogenic activity. DNA at -145 -573 of the upstream region did not increase transcription any further. DNA including 2500 bp of the upstream region stimulated transcription by 10 fold only in adrenal Y-1 cells. Hence in the -145 region contains a low level P450scc promoter and the 2500 bp DNA possesses an adrenal specific enhancing element.",

author = "Chung, {Bon chu} and Hu, {Meng Chun} and Lai, {Char Chang} and Lin, {Cheng Hui}",

note = "Funding Information: The authors would like to thank Drs. Ru-Chih discussions and encouragement and Dr. Ming oligonucleotides. This work was supported by Academia Sinica, Republic of China.",

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doi = "10.1016/0006-291X(89)91652-5",

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T1 - The 5′-region of the P450XIA1 (P450scc) gene contains a basal promoter and an adrenal-specific activating domain

AU - Chung, Bon chu

AU - Hu, Meng Chun

AU - Lai, Char Chang

AU - Lin, Cheng Hui

N1 - Funding Information: The authors would like to thank Drs. Ru-Chih discussions and encouragement and Dr. Ming oligonucleotides. This work was supported by Academia Sinica, Republic of China.

PY - 1989/4/14

Y1 - 1989/4/14

N2 - The first step to the synthesis of all steroids is catalyzed by P450scc. We constructed nine deletion mutants of the 5′-region of the P450scc gene and connected them to a CAT reporter gene to assay transcriptional activity of the P450scc promoter. A short 145 bp fragment stimulated transcriptionaby two fold. This DNA was active in all cells tested irrespective of their tissue origin and steroidogenic activity. DNA at -145 -573 of the upstream region did not increase transcription any further. DNA including 2500 bp of the upstream region stimulated transcription by 10 fold only in adrenal Y-1 cells. Hence in the -145 region contains a low level P450scc promoter and the 2500 bp DNA possesses an adrenal specific enhancing element.

AB - The first step to the synthesis of all steroids is catalyzed by P450scc. We constructed nine deletion mutants of the 5′-region of the P450scc gene and connected them to a CAT reporter gene to assay transcriptional activity of the P450scc promoter. A short 145 bp fragment stimulated transcriptionaby two fold. This DNA was active in all cells tested irrespective of their tissue origin and steroidogenic activity. DNA at -145 -573 of the upstream region did not increase transcription any further. DNA including 2500 bp of the upstream region stimulated transcription by 10 fold only in adrenal Y-1 cells. Hence in the -145 region contains a low level P450scc promoter and the 2500 bp DNA possesses an adrenal specific enhancing element.

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The 5′-region of the P450XIA1 (P450scc) gene contains a basal promoter and an adrenal-specific activating domain

Abstract

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