Th1 shift in CIDP versus Th2 shift in vasculitic neuropathy in CSF

Feng Jun Mei, Takaaki Ishizu, Hiroyuki Murai, Manabu Osoegawa, Motozumi Minohara, Kun Nan Zhang, Jun Ichi Kira

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49 Citations (Scopus)


To investigate the intra- and extracellular levels of various cytokines and chemokines in CSF in chronic inflammatory demyelinating polyneuropathy (CIDP) and vasculitic neuropathy (VN), 16 cytokines, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17, IFN-γ, TNF-α, G-CSF, MCP-1 and MIP-1β, were measured in CSF supernatant by a multiplexed fluorescent bead-based immunoassay and intracellular production of IFN-γ and IL-4 in CSF CD4 + T cells were simultaneously measured by flow cytometry in 14 patients with CIDP, 8 patients with VN and 25 patients with other noninflammatory neurologic diseases (OND). In the CSF supernatant, a significant increase of IL-17, IL-8 and IL-6, and a significant decrease of IL-4, IL-5 and IL-7 levels were detected in pretreated CIDP as compared with OND. A significant increase of IL-6, IL-8 and IL-10 levels was found in pretreated VN. Both IL-17 and IL-8 levels correlated strongly with CSF protein levels in CIDP, although the correlation of IL-6 levels was weak. In CSF CD4 + T cells, IFN-γ + IL-4 - cell percentages were markedly elevated in CIDP compared with OND, but not in VN, resulting in a significant increase of intracellular IFN-γ/IL-4 ratio in CIDP, even in the absence of CSF pleocytosis. The nonresponders to intravenous immunoglobulins (IVIGs) showed a significantly lower IFN-γ - IL-4 + CD4 + T cell percentage, and tended to have a higher intracellular IFN-γ/IL-4 ratio than the responders in CSF. Marked upregulation of Th1 cytokine, IL-17, and downregulation of Th2 cytokines, together with infiltration of IFN-γ-producing CD4 + T cells are useful markers for CIDP, while several Th2 cytokines are upregulated in VN in CSF.

Original languageEnglish
Pages (from-to)75-85
Number of pages11
JournalJournal of the Neurological Sciences
Issue number1
Publication statusPublished - Jan 15 2005

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology


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