Tetrodotoxin- and tributyltin-binding abilities of recombinant pufferfish saxitoxin and tetrodotoxin binding proteins of Takifugu rubripes

Hina Satone; Shohei Nonaka; Jae Man Lee; Yohei Shimasaki; Takahiro Kusakabe; Shun ichiro Kawabata; Yuji Oshima

doi:10.1016/j.toxicon.2016.11.245

Tetrodotoxin- and tributyltin-binding abilities of recombinant pufferfish saxitoxin and tetrodotoxin binding proteins of Takifugu rubripes

Hina Satone, Shohei Nonaka, Jae Man Lee, Yohei Shimasaki, Takahiro Kusakabe, Shun ichiro Kawabata, Yuji Oshima

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

We investigated the ability of recombinant pufferfish saxitoxin and tetrodotoxin binding protein types 1 and 2 of Takifugu rubripes (rTrub.PSTBP1 and rTrub.PSTBP2) to bind to tetrodotoxin (TTX) and tributyltin. Both rTrub.PSTBPs bound to tributyltin in an ultrafiltration binding assay but lost this ability on heat denaturation. In contrast, only rTrub.PSTBP2 bound to TTX even heat denaturation. This result suggests that the amino acid sequence of PSTBP2 may be contributed for its affinity for TTX.

Original language	English
Pages (from-to)	50-52
Number of pages	3
Journal	Toxicon
Volume	125
DOIs	https://doi.org/10.1016/j.toxicon.2016.11.245
Publication status	Published - Jan 1 2017

All Science Journal Classification (ASJC) codes

Toxicology

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10.1016/j.toxicon.2016.11.245

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title = "Tetrodotoxin- and tributyltin-binding abilities of recombinant pufferfish saxitoxin and tetrodotoxin binding proteins of Takifugu rubripes",

abstract = "We investigated the ability of recombinant pufferfish saxitoxin and tetrodotoxin binding protein types 1 and 2 of Takifugu rubripes (rTrub.PSTBP1 and rTrub.PSTBP2) to bind to tetrodotoxin (TTX) and tributyltin. Both rTrub.PSTBPs bound to tributyltin in an ultrafiltration binding assay but lost this ability on heat denaturation. In contrast, only rTrub.PSTBP2 bound to TTX even heat denaturation. This result suggests that the amino acid sequence of PSTBP2 may be contributed for its affinity for TTX.",

author = "Hina Satone and Shohei Nonaka and Lee, {Jae Man} and Yohei Shimasaki and Takahiro Kusakabe and Kawabata, {Shun ichiro} and Yuji Oshima",

note = "Funding Information: This work was supported by JSPS KAKENHI Grant Number JP20580221 . We thanks to Center for Advanced Instrumental and Educational Supports, Faculty of Agriculture, Kyushu University for technical advice for LCMS-IT-TOF analysis. ",

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doi = "10.1016/j.toxicon.2016.11.245",

language = "English",

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journal = "Toxicon",

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TY - JOUR

T1 - Tetrodotoxin- and tributyltin-binding abilities of recombinant pufferfish saxitoxin and tetrodotoxin binding proteins of Takifugu rubripes

AU - Satone, Hina

AU - Nonaka, Shohei

AU - Lee, Jae Man

AU - Shimasaki, Yohei

AU - Kusakabe, Takahiro

AU - Kawabata, Shun ichiro

AU - Oshima, Yuji

N1 - Funding Information: This work was supported by JSPS KAKENHI Grant Number JP20580221 . We thanks to Center for Advanced Instrumental and Educational Supports, Faculty of Agriculture, Kyushu University for technical advice for LCMS-IT-TOF analysis.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - We investigated the ability of recombinant pufferfish saxitoxin and tetrodotoxin binding protein types 1 and 2 of Takifugu rubripes (rTrub.PSTBP1 and rTrub.PSTBP2) to bind to tetrodotoxin (TTX) and tributyltin. Both rTrub.PSTBPs bound to tributyltin in an ultrafiltration binding assay but lost this ability on heat denaturation. In contrast, only rTrub.PSTBP2 bound to TTX even heat denaturation. This result suggests that the amino acid sequence of PSTBP2 may be contributed for its affinity for TTX.

AB - We investigated the ability of recombinant pufferfish saxitoxin and tetrodotoxin binding protein types 1 and 2 of Takifugu rubripes (rTrub.PSTBP1 and rTrub.PSTBP2) to bind to tetrodotoxin (TTX) and tributyltin. Both rTrub.PSTBPs bound to tributyltin in an ultrafiltration binding assay but lost this ability on heat denaturation. In contrast, only rTrub.PSTBP2 bound to TTX even heat denaturation. This result suggests that the amino acid sequence of PSTBP2 may be contributed for its affinity for TTX.

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SN - 0041-0101

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JF - Toxicon

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Tetrodotoxin- and tributyltin-binding abilities of recombinant pufferfish saxitoxin and tetrodotoxin binding proteins of Takifugu rubripes

Abstract

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