@inbook{8b641673f8c9415da85a7197588e4729,
title = "Targeting Two-Component Signal Transduction: A Novel Drug Discovery System",
abstract = "We have developed two screening systems for isolating inhibitors that target bacterial two-component signal transduction: (1) a differential growth assay using a temperature-sensitive yycF mutant (CNM2000) of Bacillus subtilis, which is supersensitive to histidine kinase inhibitors, and (2) a high-throughput genetic system for targeting the homodimerization of histidine kinases essential for the bacterial two-component signal transduction. By using these methods, we have been able to identify various types of inhibitors that block the autophosphorylation of histidine kinases with different modes of actions.",
author = "Ario Okada and Yasuhiro Gotoh and Takafumi Watanabe and Eiji Furuta and Kaneyoshi Yamamoto and Ryutaro Utsumi",
note = "Funding Information: The authors are grateful to Dr. K. Watabe for critical reading of this chapter. This work was supported by the Research and Development Program for New Bio‐industry Initiatives (2006–2010) from the Bio‐oriented Technology Research Advancement Institution and the Academic Frontier Project for Private Universities through a matching funds subsidy from the Ministry of Education, Culture, Sports, Science, and Technology (2004–2008). It was also supported in part by Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence, JST (2005–2009).",
year = "2007",
doi = "10.1016/S0076-6879(06)22019-6",
language = "English",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "386--395",
booktitle = "Two Component Signaling Systems, Part A",
address = "United States",
}