Targeting Two-Component Signal Transduction: A Novel Drug Discovery System

Ario Okada, Yasuhiro Gotoh, Takafumi Watanabe, Eiji Furuta, Kaneyoshi Yamamoto, Ryutaro Utsumi

Research output: Chapter in Book/Report/Conference proceedingChapter

41 Citations (Scopus)


We have developed two screening systems for isolating inhibitors that target bacterial two-component signal transduction: (1) a differential growth assay using a temperature-sensitive yycF mutant (CNM2000) of Bacillus subtilis, which is supersensitive to histidine kinase inhibitors, and (2) a high-throughput genetic system for targeting the homodimerization of histidine kinases essential for the bacterial two-component signal transduction. By using these methods, we have been able to identify various types of inhibitors that block the autophosphorylation of histidine kinases with different modes of actions.

Original languageEnglish
Title of host publicationTwo Component Signaling Systems, Part A
PublisherAcademic Press Inc.
Number of pages10
Publication statusPublished - 2007
Externally publishedYes

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology


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