TY - JOUR
T1 - T-cell subsets regulating anti-acetylcholine-receptor-antibody formation in myasthenia gravis and characterization of suppressor T-cell factors involved
AU - Shinomiya, Noriaki
AU - Yata, Junichi
AU - Sasazuki, Takehiko
N1 - Funding Information:
This work was supported in part by a grant from the Ministry of Health and Welfare and a Grant-in-Aid for Scientific Research from the Ministry of Education. Science and Culture of Japan. We thank Professor K. Hayashi for providing the AChR antigen preparation and Dr. M. Uono and his staff at Department of Neurology. Tokyo Metropolitan Hospital of Fuchu. for provision of the venous blood from MC patients.
PY - 1984/11
Y1 - 1984/11
N2 - The in vitro generation of anti-acetylcholine-receptor (AChR)-antibody-forming cells from B cells of myasthenia gravis (MG) patients occurred only in the presence of OKT4+ cells. Anti-AChR-antibody formation by lymphocytes from MG patients was suppressed by normal T cells, and the requirement of both OKT4+ and OKT8+ cells was suggested for this suppression. Culture supernatant from normal T cells stimulated with excess doses of AChR antigen, but not T cells from patients, suppressed anti-AChR-antibody formation by lymphocytes from patients. The fraction of the culture supernatant with molecular weight of 45,000 to 67,000 seemed to contain an AChR-antigen-specific and antigen-binding supressor factor with some allogeneic barrier, while the fraction with a molecular weight of 10,000 to 28,000 contained the antigen-nonspecific factor.
AB - The in vitro generation of anti-acetylcholine-receptor (AChR)-antibody-forming cells from B cells of myasthenia gravis (MG) patients occurred only in the presence of OKT4+ cells. Anti-AChR-antibody formation by lymphocytes from MG patients was suppressed by normal T cells, and the requirement of both OKT4+ and OKT8+ cells was suggested for this suppression. Culture supernatant from normal T cells stimulated with excess doses of AChR antigen, but not T cells from patients, suppressed anti-AChR-antibody formation by lymphocytes from patients. The fraction of the culture supernatant with molecular weight of 45,000 to 67,000 seemed to contain an AChR-antigen-specific and antigen-binding supressor factor with some allogeneic barrier, while the fraction with a molecular weight of 10,000 to 28,000 contained the antigen-nonspecific factor.
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U2 - 10.1016/0090-1229(84)90073-4
DO - 10.1016/0090-1229(84)90073-4
M3 - Article
C2 - 6207969
AN - SCOPUS:0021745388
SN - 0090-1229
VL - 33
SP - 182
EP - 190
JO - Clinical Immunology and Immunopathology
JF - Clinical Immunology and Immunopathology
IS - 2
ER -