TY - JOUR
T1 - Synthesis of γ-N-modified 8-oxo-2’-deoxyguanosine triphosphate and its characterization
AU - Thavoncharoensub, Ninticha
AU - Maruyama, Kento
AU - Heh, Choon Han
AU - Hoong Leong, Kok
AU - Shi, Hui
AU - Shigematsu, Yoshiharu
AU - Sasaki, Shigeki
AU - Taniguchi, Yosuke
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research (B) [JSPS KAKENHI Grant Number 16H05100]; Takeda Science Foundation; and Fukuoka Foundation for Sound Health Cancer Research Fund.
Publisher Copyright:
© 2019, © 2019 Taylor & Francis Group, LLC.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/8/3
Y1 - 2019/8/3
N2 - 8-OxodGTP is generated by the reaction between dGTP and reactive oxygen species and a considered mutagenic nucleotide. It can be incorporated into the duplex DNA during replication processes by the DNA polymerase, and thus the repair enzyme removes oxodGTP from the nucleotide pools in living cells. On the other hand, the γ-modified triphosphates show interesting properties for use as biological tools. Therefore, the γ-N-pyrenylalkyl-oxodGTP derivatives were synthesized and their effect on the enzymatic reactions were evaluated. The γ-N-pyrenylmethyl-oxodGTP was found to be accepted by the DNA polymerase just like oxodGTP, but showed a competitive inhibition property for the human oxodGTPase.
AB - 8-OxodGTP is generated by the reaction between dGTP and reactive oxygen species and a considered mutagenic nucleotide. It can be incorporated into the duplex DNA during replication processes by the DNA polymerase, and thus the repair enzyme removes oxodGTP from the nucleotide pools in living cells. On the other hand, the γ-modified triphosphates show interesting properties for use as biological tools. Therefore, the γ-N-pyrenylalkyl-oxodGTP derivatives were synthesized and their effect on the enzymatic reactions were evaluated. The γ-N-pyrenylmethyl-oxodGTP was found to be accepted by the DNA polymerase just like oxodGTP, but showed a competitive inhibition property for the human oxodGTPase.
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U2 - 10.1080/15257770.2019.1586919
DO - 10.1080/15257770.2019.1586919
M3 - Article
C2 - 30929604
AN - SCOPUS:85063631756
SN - 1525-7770
VL - 38
SP - 578
EP - 589
JO - Nucleosides, Nucleotides and Nucleic Acids
JF - Nucleosides, Nucleotides and Nucleic Acids
IS - 8
ER -