TY - GEN
T1 - Synthesis and in vitro cell compatibility of α-tricalcium phosphate-based apatite cement containing tricalcium silicate
AU - Cardenas, L. J.
AU - Takeuchi, A.
AU - Tsuru, Kanji
AU - Matsuya, S.
AU - Ishikawa, Kunio
PY - 2010/5/21
Y1 - 2010/5/21
N2 - The presence of silicate in artificial bone graft material is known to be effective in increasing bone formation rate. We previously reported the basic setting properties of α-tricalcium phosphate (α-Ca 3PO4; α-TCP) based apatite cement (AC) with various amount of tricalcium silicate (Ca3SiO5; alite) addition using sodium dihydrogen phosphate (NaH2PO4) as the liquid phase. In this study, in vitro biological compatibility of pure α-TCP and 2.5-10.0 wt% alite added to α-TCP based AC is investigated in terms of cell attachment, proliferation and differentiation. Set ACs aged for 168 hrs were used for cell studies with bone marrow cells from tibia of 4 week-old, male, SD rat. Initial cell attachment was observed after 7 hrs; cell proliferation was observed after 3, 5, and 7 days. Cell differentiation (alkaline phosphatase activity) was observed after 3, 6, and 9 days of incubation. Cell attachment of alite added ACs were almost the same as sintered hydroxyapatite (HAp) and alite-free AC. Cell proliferation at 2.5-7.5 wt% alite added ACs were significantly higher at day 5 and 7 (p<0.05). Alkaline phosphatase activity revealed that 2.5 wt% alite addition accelerated the osteogenic cell activity at day 6 and 9 (p<0.05) whereas too much alite addition resulted in the decrease of osteogenic cell activity. In conclusion, we found that α-TCP based AC containing appropriate amount of alite could be an improved AC since it shows suitable setting time, higher mechanical strength and possible higher osteoconductivity.
AB - The presence of silicate in artificial bone graft material is known to be effective in increasing bone formation rate. We previously reported the basic setting properties of α-tricalcium phosphate (α-Ca 3PO4; α-TCP) based apatite cement (AC) with various amount of tricalcium silicate (Ca3SiO5; alite) addition using sodium dihydrogen phosphate (NaH2PO4) as the liquid phase. In this study, in vitro biological compatibility of pure α-TCP and 2.5-10.0 wt% alite added to α-TCP based AC is investigated in terms of cell attachment, proliferation and differentiation. Set ACs aged for 168 hrs were used for cell studies with bone marrow cells from tibia of 4 week-old, male, SD rat. Initial cell attachment was observed after 7 hrs; cell proliferation was observed after 3, 5, and 7 days. Cell differentiation (alkaline phosphatase activity) was observed after 3, 6, and 9 days of incubation. Cell attachment of alite added ACs were almost the same as sintered hydroxyapatite (HAp) and alite-free AC. Cell proliferation at 2.5-7.5 wt% alite added ACs were significantly higher at day 5 and 7 (p<0.05). Alkaline phosphatase activity revealed that 2.5 wt% alite addition accelerated the osteogenic cell activity at day 6 and 9 (p<0.05) whereas too much alite addition resulted in the decrease of osteogenic cell activity. In conclusion, we found that α-TCP based AC containing appropriate amount of alite could be an improved AC since it shows suitable setting time, higher mechanical strength and possible higher osteoconductivity.
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U2 - 10.1007/978-3-642-12020-6-52
DO - 10.1007/978-3-642-12020-6-52
M3 - Conference contribution
AN - SCOPUS:77952402389
SN - 9783642120190
T3 - IFMBE Proceedings
SP - 207
EP - 210
BT - The Third International Conference on the Development of Biomedical Engineering in Vietnam
T2 - 3rd International Conference on the Development of Biomedical Engineering in Vietnam
Y2 - 11 January 2010 through 14 January 2010
ER -