Synthesis and evaluation of the luciferase-oligodeoxynucleotide for the sequence-selective detection of nucleic acids

Fumi Nagatsugi; Ryusuke Nakahara; Kaori Inoue; Shigeki Sasaki

doi:10.1002/ardp.200800031

Synthesis and evaluation of the luciferase-oligodeoxynucleotide for the sequence-selective detection of nucleic acids

Fumi Nagatsugi, Ryusuke Nakahara, Kaori Inoue, Shigeki Sasaki

Chemo-Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

A new method for the synthesis of ODN-luciferase conjugate was investigated as a signal-amplifying sensor of the target nucleic acids. The conjugation of the luciferase was successfully achieved between the cysteine residue and the 2-amino-6-vinylpurine nucleoside of the ODN probe without significant inactivation of luciferase. The ODN-luciferase conjugate modified with PEG retained the luciferase activity and selectivity during the hybridization with the target ODN.

Original language	English
Pages (from-to)	562-567
Number of pages	6
Journal	Archiv der Pharmazie
Volume	341
Issue number	9
DOIs	https://doi.org/10.1002/ardp.200800031
Publication status	Published - Sept 2008

All Science Journal Classification (ASJC) codes

Pharmaceutical Science
Drug Discovery

Access to Document

10.1002/ardp.200800031

Cite this

@article{e1bd88c5c39443ea87bab707b092ec7f,

title = "Synthesis and evaluation of the luciferase-oligodeoxynucleotide for the sequence-selective detection of nucleic acids",

abstract = "A new method for the synthesis of ODN-luciferase conjugate was investigated as a signal-amplifying sensor of the target nucleic acids. The conjugation of the luciferase was successfully achieved between the cysteine residue and the 2-amino-6-vinylpurine nucleoside of the ODN probe without significant inactivation of luciferase. The ODN-luciferase conjugate modified with PEG retained the luciferase activity and selectivity during the hybridization with the target ODN.",

author = "Fumi Nagatsugi and Ryusuke Nakahara and Kaori Inoue and Shigeki Sasaki",

year = "2008",

month = sep,

doi = "10.1002/ardp.200800031",

language = "English",

volume = "341",

pages = "562--567",

journal = "Archiv der Pharmazie",

issn = "0365-6233",

publisher = "Wiley-VCH Verlag",

number = "9",

}

TY - JOUR

T1 - Synthesis and evaluation of the luciferase-oligodeoxynucleotide for the sequence-selective detection of nucleic acids

AU - Nagatsugi, Fumi

AU - Nakahara, Ryusuke

AU - Inoue, Kaori

AU - Sasaki, Shigeki

PY - 2008/9

Y1 - 2008/9

N2 - A new method for the synthesis of ODN-luciferase conjugate was investigated as a signal-amplifying sensor of the target nucleic acids. The conjugation of the luciferase was successfully achieved between the cysteine residue and the 2-amino-6-vinylpurine nucleoside of the ODN probe without significant inactivation of luciferase. The ODN-luciferase conjugate modified with PEG retained the luciferase activity and selectivity during the hybridization with the target ODN.

AB - A new method for the synthesis of ODN-luciferase conjugate was investigated as a signal-amplifying sensor of the target nucleic acids. The conjugation of the luciferase was successfully achieved between the cysteine residue and the 2-amino-6-vinylpurine nucleoside of the ODN probe without significant inactivation of luciferase. The ODN-luciferase conjugate modified with PEG retained the luciferase activity and selectivity during the hybridization with the target ODN.

UR - http://www.scopus.com/inward/record.url?scp=53549097908&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=53549097908&partnerID=8YFLogxK

U2 - 10.1002/ardp.200800031

DO - 10.1002/ardp.200800031

M3 - Article

C2 - 18618490

AN - SCOPUS:53549097908

SN - 0365-6233

VL - 341

SP - 562

EP - 567

JO - Archiv der Pharmazie

JF - Archiv der Pharmazie

IS - 9

ER -

Synthesis and evaluation of the luciferase-oligodeoxynucleotide for the sequence-selective detection of nucleic acids

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this