Synthesis and characterization of polysaccharide-oligonucleotide conjugate

Naoya Fukuda; Naohiko Shimada; Seiji Shinkai; Kazuo Sakurai

Synthesis and characterization of polysaccharide-oligonucleotide conjugate

Naoya Fukuda, Naohiko Shimada, Seiji Shinkai, Kazuo Sakurai

Research output: Contribution to conference › Paper › peer-review

Abstract

We have reported that SPG can from a complex with oligodeoxynucleotide (ODN) attaching (<A₂₀) tail at 3′ end. However, SPG cannot form the complex with ODN attaching short tail (<dAso) due to reduction of thermodynamic stability. In this study, we examined whether ODN-conjugated SPG can form an intramolecular complex. dA₂₀ was introduced to reducing terminal of SPG via diaminoalkanes or diaminoPEGs. The conjugates were purified by gel-filtration. CD spectrum of the conjugate indicated a formation of intramolecular complex.

Original language	English
Pages	4828
Number of pages	1
Publication status	Published - 2006
Externally published	Yes
Event	55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan Duration: Sept 20 2006 → Sept 22 2006

Other

Other	55th Society of Polymer Science Japan Symposium on Macromolecules
Country/Territory	Japan
City	Toyama
Period	9/20/06 → 9/22/06

All Science Journal Classification (ASJC) codes

Engineering(all)

Cite this

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title = "Synthesis and characterization of polysaccharide-oligonucleotide conjugate",

abstract = "We have reported that SPG can from a complex with oligodeoxynucleotide (ODN) attaching (<A20) tail at 3′ end. However, SPG cannot form the complex with ODN attaching short tail (20 was introduced to reducing terminal of SPG via diaminoalkanes or diaminoPEGs. The conjugates were purified by gel-filtration. CD spectrum of the conjugate indicated a formation of intramolecular complex.",

author = "Naoya Fukuda and Naohiko Shimada and Seiji Shinkai and Kazuo Sakurai",

year = "2006",

language = "English",

pages = "4828",

note = "55th Society of Polymer Science Japan Symposium on Macromolecules ; Conference date: 20-09-2006 Through 22-09-2006",

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TY - CONF

T1 - Synthesis and characterization of polysaccharide-oligonucleotide conjugate

AU - Fukuda, Naoya

AU - Shimada, Naohiko

AU - Shinkai, Seiji

AU - Sakurai, Kazuo

PY - 2006

Y1 - 2006

N2 - We have reported that SPG can from a complex with oligodeoxynucleotide (ODN) attaching (<A20) tail at 3′ end. However, SPG cannot form the complex with ODN attaching short tail (20 was introduced to reducing terminal of SPG via diaminoalkanes or diaminoPEGs. The conjugates were purified by gel-filtration. CD spectrum of the conjugate indicated a formation of intramolecular complex.

AB - We have reported that SPG can from a complex with oligodeoxynucleotide (ODN) attaching (<A20) tail at 3′ end. However, SPG cannot form the complex with ODN attaching short tail (20 was introduced to reducing terminal of SPG via diaminoalkanes or diaminoPEGs. The conjugates were purified by gel-filtration. CD spectrum of the conjugate indicated a formation of intramolecular complex.

UR - http://www.scopus.com/inward/record.url?scp=33846137023&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846137023&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33846137023

SP - 4828

T2 - 55th Society of Polymer Science Japan Symposium on Macromolecules

Y2 - 20 September 2006 through 22 September 2006

ER -

Synthesis and characterization of polysaccharide-oligonucleotide conjugate

Abstract

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All Science Journal Classification (ASJC) codes

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