Synthesis and biological activity of 2-aminothiazolines and 2-mercaptothiazolines as octopaminergic agonists

Akinori Hirashima, Yutaka Yoshii, Morifusa Eto

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30 Citations (Scopus)


2-Aminothiazoline derivatives were synthesized by both hydrochloric acid-catalyzed cyclization of thiourea and by cyclization of β-aminoalkyl hydrogen sulfate with isothiocyanate in the presence of sodium hydroxide. Substituted 2-mercaptothiazoline derivatives were prepared by alkylation or acylation of the sodium salt of 2-mercaptothiazoline, which was obtained from β-aminoalkyl hydrogen sulfate with carbon disulfide. 2-(4-Chloro-O-toluidino)-2-thiazoline (III-16) was 33% as effective as octopamine at 100 μM in stimulating adenylate cyclase of Periplaneta americana ventral-nerve-cord homogenates. Its activity was nonadditive to the activity of octopamine. Stimulation of nerve-cord adenylate-cyclase activity by III-16 was inhibited by several antagonists, including mianserin, cyproheptadine, chlorpromazine and gramine. The rank-order ability of these antagonists to block the activation by III-16 was identical to the rank-order ability of the same antagonists to block enzyme activation by octopamine. The β-adrenergic antagonist propranolol was less potent. These data suggest that III-16 is a potent and selective agonist of octopamine-activated adenylate cyclase. Aminothiazolines which activated adenylate cyclase by 10–87% relative to octopamine also had acaricidal activity at 300 ppm, indicating a correlation between the in vitro octopaminergic-agonist activity and in vivo acaricidal activity of aminothiazolines.

Original languageEnglish
Pages (from-to)2537-2545
Number of pages9
JournalAgricultural and Biological Chemistry
Issue number10
Publication statusPublished - Oct 1991

All Science Journal Classification (ASJC) codes

  • General Biochemistry,Genetics and Molecular Biology
  • General Agricultural and Biological Sciences


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