Synergistic extraction of nucleobases by the combination of calixarene and D2EHPA

Kojiro Shimojo, Masahiro Goto

    Research output: Contribution to journalArticlepeer-review

    14 Citations (Scopus)


    A calix[6]arene carboxylic acid derivative incorporating tert-octyl groups at the upper rim was found to be soluble in an aliphatic organic solvent. The extraction behavior of various nucleobases was investigated with the calix[6]arene carboxylic acid derivative. In an isooctane system, neither calix[6]arene nor D2EHPA were very effective at nucleobase extraction. However, addition of D2EHPA along with calix[6]arene drastically enhanced the efficiency of adenine and cytosine extraction. It was found that D2EHPA is a powerful synergistic reagent with calix[6]arene for adenine extraction. It was demonstrated that one nucleobases forms a stable complex with one calix[6]arene and two D2EHPA molecules, based on Job's method. Adenine was recovered from the organic phase by contacting with a fresh acidic solution.

    Original languageEnglish
    Pages (from-to)175-180
    Number of pages6
    JournalSeparation and Purification Technology
    Issue number2
    Publication statusPublished - Jul 15 2005

    All Science Journal Classification (ASJC) codes

    • Analytical Chemistry
    • Filtration and Separation


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