Survival and differentiation of embryonic neural explants on different biomaterials

José Miguel Soria, Cristina Martínez Ramos, Manuel Salmerón Sánchez, Veronique Benavent, Alberto Campillo Fernández, José Luis Gómez Ribelles, José Manuel García Verdugo, Manuel Monleón Pradas, Juan Antonio Barcia

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)


Biomaterials prepared from polyacrylamide, ethyl acrylate (EA), and hydroxyethyl acrylate (HEA) in various blend ratios, methyl acrylate and chitosan, were tested in vitro as culture substrates and compared for their ability to be colonized by the cells migrating from embryonic brain explants. Neural explants were isolated from proliferative areas of the medial ganglionic eminence and the cortical ventricular zone of embryonic rat brains and cultured in vitro on the different biomaterials. Chitosan, poly(methyl acrylate), and the 50% wt copolymer of EA and HEA were the most suitable substrates to promote cell attachment and differentiation of the neural cells among those tested. Immunofluorescence microscopy analysis showed that progenitor cells had undergone differentiation and that the resulting glial and neuronal cells expressed their intrinsic morphological characteristics in culture.

Original languageEnglish
Pages (from-to)495-502
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Issue number3
Publication statusPublished - Dec 1 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys


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