Survey of solasodine-type glycoalkaloids by western blotting and ELISA using anti-solamargine monoclonal antibody

Waraporn Putalun, Hiroyuki Tanaka, Shoji Yahara, Sorasak Lhieochaiphan, Yukihiro Shoyama

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Solasodine glycosides were analyzed for the selection of higher- producing natural resources in the fruits of Solanum spp. by western blotting and ELISA using anti-solamargine monoclonal antibody (MAb). Western blotting of Solanum khasianum showed a correlation with the analysis by ELISA. The results indicated that the fruits of S. khasianum contained the highest level of solasodine glycosides, compared to other species having 53.43±3.90 μg/mg dry wt. The high level of solasodine glycosides in S. khasianum suggests that it may be suitable for commercial production.

Original languageEnglish
Pages (from-to)72-75
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume23
Issue number1
DOIs
Publication statusPublished - Jan 2000

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

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