TY - JOUR
T1 - Surface plasmon resonance-based detection of ladder-shaped polyethers by inhibition detection method
AU - Mouri, Ryota
AU - Oishi, Tohru
AU - Torikai, Kohei
AU - Ujihara, Satoru
AU - Matsumori, Nobuaki
AU - Murata, Michio
AU - Oshima, Yasukatsu
N1 - Funding Information:
We are grateful to Toshiyuki Yamaguchi in our laboratory for discussions and measuring NMR spectra. This work was supported by Grants-in-Aid for Scientific Research (S) (No. 18101010), for Priority Area (A) (No. 16073211), and for Young Scientists (A) (No. 17681027) from MEXT, Japan. A research fellowship to S. U. from GCOE program, ‘Global Education and Research Center for Bio-Environmental Chemistry’, is also acknowledged.
PY - 2009/5/15
Y1 - 2009/5/15
N2 - Ladder-shaped polyether (LSP) compounds represented by brevetoxins and ciguatoxins were largely discovered in association with seafood poisoning. Thus, a quick quantification method for LSPs is potentially important. We examined a surface plasmon resonance method using desulfated-yessotoxin (dsYTX) immobilized on a sensor chip and phosphodiesterase PDEII in a inhibition detection mode. Yessotoxin, brevetoxin B and synthetic LSP derivatives showed clear inhibition against PDEII binding to the immobilized dsYTX, by which their half inhibitory concentrations were successfully estimated. This inhibition method appeared to be superior in specificity to direct binding assays where binding proteins to LSP was immobilized on a sensor chip.
AB - Ladder-shaped polyether (LSP) compounds represented by brevetoxins and ciguatoxins were largely discovered in association with seafood poisoning. Thus, a quick quantification method for LSPs is potentially important. We examined a surface plasmon resonance method using desulfated-yessotoxin (dsYTX) immobilized on a sensor chip and phosphodiesterase PDEII in a inhibition detection mode. Yessotoxin, brevetoxin B and synthetic LSP derivatives showed clear inhibition against PDEII binding to the immobilized dsYTX, by which their half inhibitory concentrations were successfully estimated. This inhibition method appeared to be superior in specificity to direct binding assays where binding proteins to LSP was immobilized on a sensor chip.
UR - http://www.scopus.com/inward/record.url?scp=65349114284&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65349114284&partnerID=8YFLogxK
U2 - 10.1016/j.bmcl.2009.03.103
DO - 10.1016/j.bmcl.2009.03.103
M3 - Article
C2 - 19361990
AN - SCOPUS:65349114284
SN - 0960-894X
VL - 19
SP - 2824
EP - 2828
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 10
ER -