TY - JOUR
T1 - Surface modification of gold nanorods using layer-by-layer technique for cellular uptake
AU - Takahashi, Hironobu
AU - Niidome, Takuro
AU - Kawano, Takahito
AU - Yamada, Sunao
AU - Niidome, Yasuro
N1 - Funding Information:
Acknowledgments This study was supported in part by Research Fellowships of the Japan Society for Promotion of Science (JSPS) for Young Scientists, and by a Grant-in-Aid for Scientific Research (KAKENHI) in the Priority Area ‘‘Molecular Nano Dynamics’’ from the Ministry of Education, Culture, Sports, Science and Technology.
PY - 2008/2
Y1 - 2008/2
N2 - Gold nanorods (NRs), rod-shaped gold nanoparticles, were modified with bovine serum albumin (BSA) and polyethylenimine (PEI) using layer-by-layer technique. From absorption spectroscopy and zeta potential measurements, it was obvious that NRs were wrapped with these polymers without aggregation of NRs. Following BSA modification, the surface-modified NRs (BSA-NRs) were well-dispersed without aggregation in biochemical conditions, verified from absorption spectroscopy. Further modification with PEI provided positively charged NRs (PEI-NRs). A transmission electron microscopy image of PEI-NRs revealed that the surface modification did not affect changing the shape of the initial NRs. In addition, the PEI-NRs retained the colloidal stability of BSA-NRs in biochemical conditions. We have evaluated that transfection activity of PEI-NRs with HeLa cells. From results of gene expression experiments, it was obvious that the stabilization of NRs by BSA and further modification with PEI realized transfection using NRs into cultured cells. Moreover, the cellular uptake of NRs enabled cellular imaging using light scattering from the NRs.
AB - Gold nanorods (NRs), rod-shaped gold nanoparticles, were modified with bovine serum albumin (BSA) and polyethylenimine (PEI) using layer-by-layer technique. From absorption spectroscopy and zeta potential measurements, it was obvious that NRs were wrapped with these polymers without aggregation of NRs. Following BSA modification, the surface-modified NRs (BSA-NRs) were well-dispersed without aggregation in biochemical conditions, verified from absorption spectroscopy. Further modification with PEI provided positively charged NRs (PEI-NRs). A transmission electron microscopy image of PEI-NRs revealed that the surface modification did not affect changing the shape of the initial NRs. In addition, the PEI-NRs retained the colloidal stability of BSA-NRs in biochemical conditions. We have evaluated that transfection activity of PEI-NRs with HeLa cells. From results of gene expression experiments, it was obvious that the stabilization of NRs by BSA and further modification with PEI realized transfection using NRs into cultured cells. Moreover, the cellular uptake of NRs enabled cellular imaging using light scattering from the NRs.
UR - http://www.scopus.com/inward/record.url?scp=36448988583&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36448988583&partnerID=8YFLogxK
U2 - 10.1007/s11051-007-9227-5
DO - 10.1007/s11051-007-9227-5
M3 - Article
AN - SCOPUS:36448988583
SN - 1388-0764
VL - 10
SP - 221
EP - 228
JO - Journal of Nanoparticle Research
JF - Journal of Nanoparticle Research
IS - 1
ER -