Suppression of neural stem cell differentiation by Honeycomb-patterned films

Akinori Tsuruma, Masaru Tanaka, Sada Aki Yamamoto, Masatsugu Shimomura

Research output: Contribution to journalConference articlepeer-review


Neural stem cells (NSCs) play important roles in brain development. NSCs have self-renewal potency and can differentiate into phenotypes of neural cells (neuron, astrocyte and oligodendrocyte). Recently, NSCs are expected for regenerative medicine to be transplanted into damaged neural tissues. For this purpose, it is important to maintain these properties of NSCs in culture. Nano-micro patterns on the surface of scaffold have significant influences on the cell morphologies, proliferation, differentiation and function. In previous study, we prepared highly regular porous films (honeycomb-patterned films) by a casting technique, and investigated neural cell morphologies on flat film and honeycomb-patterned films. As a result, morphologies of adhered neural cells were changed by varying pore size of the honeycomb-patterned films. Especially, we found that cell aggregates were formed on the patterned films of pore size 3 m. Our current study showed that NSCs were suppressed its differentiation into neurons on the patterned films of pore size 3 μm. This result indicate that NSCs are positively regulated their own survival and differentiation. To use neural stem cells / progenitor cells for regenerative medicine, we need environments where these NSCs can live in vitro without losing their ability to proliferation and differentiation.

Original languageEnglish
Pages (from-to)3558-3561
Number of pages4
JournalIFMBE Proceedings
Issue number1
Publication statusPublished - Jan 1 2007
Externally publishedYes
Event10th World Congress on Medical Physics and Biomedical Engineering, WC 2006 - Seoul, Korea, Republic of
Duration: Aug 27 2006Sept 1 2006

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomedical Engineering


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