Suppression of immunoglobulin production in human peripheral blood mononuclear cells by monocytes via secretion of heavy-chain ferritin

Makiko Yamashita; Gakuro Harada; Shin ei Matsumoto; Yoshihiro Aiba; Akira Ichikawa; Tsukasa Fujiki; Miyako Udono; Shigeru Kabayama; Tadashi Yoshida; Pingbo Zhang; Hiroshi Fujii; Sanetaka Shirahata; Yoshinori Katakura

doi:10.1016/j.imbio.2013.08.011

Suppression of immunoglobulin production in human peripheral blood mononuclear cells by monocytes via secretion of heavy-chain ferritin

Makiko Yamashita, Gakuro Harada, Shin ei Matsumoto, Yoshihiro Aiba, Akira Ichikawa, Tsukasa Fujiki, Miyako Udono, Shigeru Kabayama, Tadashi Yoshida, Pingbo Zhang, Hiroshi Fujii, Sanetaka Shirahata, Yoshinori Katakura

Systems Biology

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

In vitro antigen stimulation of peripheral blood mononuclear cells (PBMCs) does not induce immunoglobulin (Ig) production. However, pretreatment of PBMCs with l-leucyl-l-leucine methyl ester (LLME) prior to in vitro stimulation removes the suppression of Ig production. In the present study, we attempted to identify the target cells of LLME and determine the mechanisms by which Ig production in PBMCs is suppressed. We found that CD14⁺ monocytes are involved in the suppression of Ig production in PBMCs. Furthermore, we confirmed that heavy-chain ferritin derived from CD14⁺ monocytes suppresses Ig production in PBMCs, possibly through iron sequestration.

Original language	English
Pages (from-to)	149-157
Number of pages	9
Journal	Immunobiology
Volume	219
Issue number	2
DOIs	https://doi.org/10.1016/j.imbio.2013.08.011
Publication status	Published - Feb 2014

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology
Hematology

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Yamashita, M., Harada, G., Matsumoto, S. E., Aiba, Y., Ichikawa, A., Fujiki, T., Udono, M., Kabayama, S., Yoshida, T., Zhang, P., Fujii, H., Shirahata, S., & Katakura, Y. (2014). Suppression of immunoglobulin production in human peripheral blood mononuclear cells by monocytes via secretion of heavy-chain ferritin. Immunobiology, 219(2), 149-157. https://doi.org/10.1016/j.imbio.2013.08.011

Yamashita, M, Harada, G, Matsumoto, SE, Aiba, Y, Ichikawa, A, Fujiki, T, Udono, M, Kabayama, S, Yoshida, T, Zhang, P, Fujii, H, Shirahata, S & Katakura, Y 2014, 'Suppression of immunoglobulin production in human peripheral blood mononuclear cells by monocytes via secretion of heavy-chain ferritin', Immunobiology, vol. 219, no. 2, pp. 149-157. https://doi.org/10.1016/j.imbio.2013.08.011

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title = "Suppression of immunoglobulin production in human peripheral blood mononuclear cells by monocytes via secretion of heavy-chain ferritin",

abstract = "In vitro antigen stimulation of peripheral blood mononuclear cells (PBMCs) does not induce immunoglobulin (Ig) production. However, pretreatment of PBMCs with l-leucyl-l-leucine methyl ester (LLME) prior to in vitro stimulation removes the suppression of Ig production. In the present study, we attempted to identify the target cells of LLME and determine the mechanisms by which Ig production in PBMCs is suppressed. We found that CD14+ monocytes are involved in the suppression of Ig production in PBMCs. Furthermore, we confirmed that heavy-chain ferritin derived from CD14+ monocytes suppresses Ig production in PBMCs, possibly through iron sequestration.",

author = "Makiko Yamashita and Gakuro Harada and Matsumoto, {Shin ei} and Yoshihiro Aiba and Akira Ichikawa and Tsukasa Fujiki and Miyako Udono and Shigeru Kabayama and Tadashi Yoshida and Pingbo Zhang and Hiroshi Fujii and Sanetaka Shirahata and Yoshinori Katakura",

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doi = "10.1016/j.imbio.2013.08.011",

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T1 - Suppression of immunoglobulin production in human peripheral blood mononuclear cells by monocytes via secretion of heavy-chain ferritin

AU - Yamashita, Makiko

AU - Harada, Gakuro

AU - Matsumoto, Shin ei

AU - Aiba, Yoshihiro

AU - Ichikawa, Akira

AU - Fujiki, Tsukasa

AU - Udono, Miyako

AU - Kabayama, Shigeru

AU - Yoshida, Tadashi

AU - Zhang, Pingbo

AU - Fujii, Hiroshi

AU - Shirahata, Sanetaka

AU - Katakura, Yoshinori

PY - 2014/2

Y1 - 2014/2

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AB - In vitro antigen stimulation of peripheral blood mononuclear cells (PBMCs) does not induce immunoglobulin (Ig) production. However, pretreatment of PBMCs with l-leucyl-l-leucine methyl ester (LLME) prior to in vitro stimulation removes the suppression of Ig production. In the present study, we attempted to identify the target cells of LLME and determine the mechanisms by which Ig production in PBMCs is suppressed. We found that CD14+ monocytes are involved in the suppression of Ig production in PBMCs. Furthermore, we confirmed that heavy-chain ferritin derived from CD14+ monocytes suppresses Ig production in PBMCs, possibly through iron sequestration.

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JO - Immunobiology

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Suppression of immunoglobulin production in human peripheral blood mononuclear cells by monocytes via secretion of heavy-chain ferritin

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