Supercritical fluid chromatography/mass spectrometry in metabolite analysis

Kaori Taguchi; Eiichiro Fukusaki; Takeshi Bamba

doi:10.4155/bio.14.120

Supercritical fluid chromatography/mass spectrometry in metabolite analysis

Kaori Taguchi, Eiichiro Fukusaki, Takeshi Bamba

Research output: Contribution to journal › Review article › peer-review

30 Citations (Scopus)

Abstract

Supercritical fluid chromatography (SFC) owes many of its advantages to the properties of supercritical CO₂, which possesses benefits as mobile phase. SFC has recently gained attention as a separation technique because it can be utilized for not only non-polar but also polar compound analysis. In addition, MS is widely adopted for SFC, and the options for MS are equivalent to liquid chromatography. Sensitive and selective detection is crucial in metabolite analysis. The SFC/MS system can be an alternative approach to liquid chromatography, as can metabolite analysis using packed-column SFC in biosamples. In this review we cover the fundamentals of SFC in combination with MS, and discuss the results of metabolite analysis using SFC/MS.

Original language	English
Pages (from-to)	1679-1689
Number of pages	11
Journal	Bioanalysis
Volume	6
Issue number	12
DOIs	https://doi.org/10.4155/bio.14.120
Publication status	Published - Jun 2014
Externally published	Yes

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Pharmacology, Toxicology and Pharmaceutics(all)
Clinical Biochemistry
Medical Laboratory Technology

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10.4155/bio.14.120

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abstract = "Supercritical fluid chromatography (SFC) owes many of its advantages to the properties of supercritical CO2, which possesses benefits as mobile phase. SFC has recently gained attention as a separation technique because it can be utilized for not only non-polar but also polar compound analysis. In addition, MS is widely adopted for SFC, and the options for MS are equivalent to liquid chromatography. Sensitive and selective detection is crucial in metabolite analysis. The SFC/MS system can be an alternative approach to liquid chromatography, as can metabolite analysis using packed-column SFC in biosamples. In this review we cover the fundamentals of SFC in combination with MS, and discuss the results of metabolite analysis using SFC/MS.",

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Supercritical fluid chromatography/mass spectrometry in metabolite analysis

Abstract

All Science Journal Classification (ASJC) codes

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