TY - GEN
T1 - Sub-millisecond separation of DNA and micro-RNA by nanopillar array chips
AU - Wu, Qiong
AU - Motoyama, Koki
AU - Yasui, Takao
AU - Rahong, Sakon
AU - Yanagida, Takeshi
AU - Kanai, Masaki
AU - Okamoto, Yukihiro
AU - Kaji, Noritada
AU - Tokeshi, Manabu
AU - Nagashima, Kazuki
AU - Kawai, Tomoji
AU - Baba, Yoshinobu
PY - 2012
Y1 - 2012
N2 - In this study, we fabricated a novel nanostructure that combined the pillar structures (nanopillar) and dammed structures (nanoslit) in nanometer-scale inside microchannels to realize ultra-fast separation. Nanopilliar chips were fabricated by using electron-beam lithography, photolithography and plasma etching. We used the nanopillar chip to realize ultra-fast separation of T4 DNA (165.5 kbp) and microRNA (22 b). By decreasing height down to 100 nm, we succeeded in separating DNA and microRNA in sub-milliseconds.
AB - In this study, we fabricated a novel nanostructure that combined the pillar structures (nanopillar) and dammed structures (nanoslit) in nanometer-scale inside microchannels to realize ultra-fast separation. Nanopilliar chips were fabricated by using electron-beam lithography, photolithography and plasma etching. We used the nanopillar chip to realize ultra-fast separation of T4 DNA (165.5 kbp) and microRNA (22 b). By decreasing height down to 100 nm, we succeeded in separating DNA and microRNA in sub-milliseconds.
UR - http://www.scopus.com/inward/record.url?scp=84901775390&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901775390&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901775390
SN - 9780979806452
T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
SP - 599
EP - 601
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Y2 - 28 October 2012 through 1 November 2012
ER -