Study on nanoparticles sizing based on fluorescence polarization (2nd report) - A novel nanopartìcle sizing method based on rotational diffusion coefficient for fluorescent DNA probe - A n

The nanopartìcle is key materials in the area of nanotechnology, and there is a strong demand to measure the particle size accurately and easily. We proposed a novel nanoparticle sizing method based on fluorescence polarization analysis, and constructed the rotational diffusion coefficient measurement system using fluorescent DNA probe (fl-DNA). Nano particle sizing is achieved by measuring the rotational diffusion coefficient of fl-DNA, which is labeled to nanoparticle. In this report, we investigate the relation between the rotational diffusion coefficient of fl-DNA and the size of the nanoparticles standard. The rotational diffusion coefficients of gold nanoparticles of diameters from 6 nm to 20 nm were measured using the proposed method.