TY - JOUR
T1 - Structural modification of NADPH oxidase activator (Noxa 1) by oxidative stress
T2 - An experimental and computational study
AU - Attri, Pankaj
AU - Park, Jae-Hyun
AU - De Backer, Joey
AU - Kim, Myeongkyu
AU - Yun, Ji-Hye
AU - Heo, Yunseok
AU - Dewilde, Sylvia
AU - Shiratani, Masaharu
AU - Choi, Eun Ha
AU - Lee, Weontae
AU - Bogaerts, Annemie
N1 - Copyright © 2020. Published by Elsevier B.V.
PY - 2020/9/19
Y1 - 2020/9/19
N2 - NADPH oxidases 1 (NOX1) derived reactive oxygen species (ROS) play an important role in the progression of cancer through signaling pathways. Therefore, in this paper, we demonstrate the effect of cold atmospheric plasma (CAP) on the structural changes of Noxa1 SH3 protein, one of the regulatory subunits of NOX1. For this purpose, firstly we purified the Noxa1 SH3 protein and analyzed the structure using X-ray crystallography, and subsequently, we treated the protein with two types of CAP reactors such as pulsed dielectric barrier discharge (DBD) and Soft Jet for different time intervals. The structural deformation of Noxa1 SH3 protein was analyzed by various experimental methods (circular dichroism, fluorescence, and NMR spectroscopy) and by MD simulations. Additionally, we demonstrate the effect of CAP (DBD and Soft Jet) on the viability and expression of NOX1 in A375 cancer cells. Our results are useful to understand the structural modification/oxidation occur in protein due to reactive oxygen and nitrogen (RONS) species generated by CAP.
AB - NADPH oxidases 1 (NOX1) derived reactive oxygen species (ROS) play an important role in the progression of cancer through signaling pathways. Therefore, in this paper, we demonstrate the effect of cold atmospheric plasma (CAP) on the structural changes of Noxa1 SH3 protein, one of the regulatory subunits of NOX1. For this purpose, firstly we purified the Noxa1 SH3 protein and analyzed the structure using X-ray crystallography, and subsequently, we treated the protein with two types of CAP reactors such as pulsed dielectric barrier discharge (DBD) and Soft Jet for different time intervals. The structural deformation of Noxa1 SH3 protein was analyzed by various experimental methods (circular dichroism, fluorescence, and NMR spectroscopy) and by MD simulations. Additionally, we demonstrate the effect of CAP (DBD and Soft Jet) on the viability and expression of NOX1 in A375 cancer cells. Our results are useful to understand the structural modification/oxidation occur in protein due to reactive oxygen and nitrogen (RONS) species generated by CAP.
U2 - 10.1016/j.ijbiomac.2020.09.120
DO - 10.1016/j.ijbiomac.2020.09.120
M3 - Article
C2 - 32961197
SN - 0141-8130
VL - 163
SP - 2405
EP - 2414
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -
