TY - JOUR
T1 - Structural elucidation of an asparagine-linked oligosaccharide from the hyperthermophilic archaeon, Pyrococcus furiosus
AU - Fujinami, Daisuke
AU - Matsumoto, Masaki
AU - Noguchi, Takuya
AU - Sonomoto, Kenji
AU - Kohda, Daisuke
N1 - Funding Information:
We thank Prof. Shinsuke Sando and Dr. Hiroshi Nonaka (Soft Materials Research Division, Inamori Frontier Research Center, Kyushu University) for the preparation of hydrochloric acid in butanol. This work was supported by JSPS KAKENHI Grants ( 21121003 and 24370047 ) to D.K.
PY - 2014/3/31
Y1 - 2014/3/31
N2 - An oligosaccharide chain attached to the asparagine residue in a structurally defined peptide was produced by an in vitro oligosaccharide- transfer reaction, using membrane fractions that contained the oligosaccharyltransferase from the hyperthermophilic archaeon, Pyrococcus furiosus. The chemical structure of the N-glycan was elucidated by sugar analysis, NMR spectroscopy, and MS spectrometry, which revealed the structure. The shorter glycan structures lacking one or two xylose residues were also transferred by the P. furiosus oligosaccharyltransferase. The archaeal N-glycans are known to exhibit a high degree of structural variation. The structure of the P. furiosus N-glycan is novel and unique. The present data will be useful for structural and functional studies of the P. furiosus oligosaccharyltransferase.
AB - An oligosaccharide chain attached to the asparagine residue in a structurally defined peptide was produced by an in vitro oligosaccharide- transfer reaction, using membrane fractions that contained the oligosaccharyltransferase from the hyperthermophilic archaeon, Pyrococcus furiosus. The chemical structure of the N-glycan was elucidated by sugar analysis, NMR spectroscopy, and MS spectrometry, which revealed the structure. The shorter glycan structures lacking one or two xylose residues were also transferred by the P. furiosus oligosaccharyltransferase. The archaeal N-glycans are known to exhibit a high degree of structural variation. The structure of the P. furiosus N-glycan is novel and unique. The present data will be useful for structural and functional studies of the P. furiosus oligosaccharyltransferase.
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U2 - 10.1016/j.carres.2014.01.021
DO - 10.1016/j.carres.2014.01.021
M3 - Article
C2 - 24562177
AN - SCOPUS:84896709172
SN - 0008-6215
VL - 387
SP - 30
EP - 36
JO - Carbohydrate Research
JF - Carbohydrate Research
IS - 1
ER -