Stress relaxation measurement of fibroblast cells with atomic force microscopy

Takaharu Okajima, Masara Tanaka, Shusaku Tsukiyama, Tsubasa Kadowaki, Sadaaki Yamamoto, Masatsugu Shimomura, Hiroshi Tokumoto

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


We measured the stress relaxation of mouse fibroblast NIH3T3 cells with an atomic force microscope (AFM) using a sharp silicon tip and a silica bead with a radius of ∼1 μm as an indenter. The decay of loading force was clearly observed in NIH3T3 cells at a small initial loading force of ∼0.4 nN and was well fitted to the stretched exponential function rather than to a single exponential function. The stretching exponent parameter was ∼0.5 for both indenters, indicating that the stress relaxation observed in NIH3T3 cells consisted of multiple relaxation processes. The time-domain AFM technique described in this report allows us to measure directly the relaxation process of living cells in a range from milliseconds to seconds.

Original languageEnglish
Pages (from-to)5552-5555
Number of pages4
JournalJapanese Journal of Applied Physics
Issue number8 B
Publication statusPublished - Aug 23 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Engineering(all)
  • Physics and Astronomy(all)


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