TY - JOUR
T1 - Stress-induced Changes in the Biogenic Amine Levels and Larval Growth of Tribolium Castaneum Herbst
AU - Nagano, Takeshi
AU - Hirashima, Akinori
AU - Eto, Morifusa
N1 - Funding Information:
Acknowledgments. We thank Dr, Masahiko Kuwahara of Tropical Agriculture Research Center and Mr. Hiroshi Fujii of National Food Research Institute for the supply of T. castaneum, and Messrs. Akira Komatsu Teruyuki Hirose, and Narumi Kasawara of Body Sonic Co. for presentin~ transducers and giving advice on assembling the vibration . apparatus. This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan.
PY - 1993
Y1 - 1993
N2 - Such stress factors as mechanical (vibration), thermal (unfavorable temperature), optical (light), and starvation reduced the larval growth of the red flour beetle (Tribolium castaneum Herbst). Various biogenic amines, including octopamine (OA), dopamine (DA), serotonin (5-HT), epinephrine (E), norepinephrine (NE), their precursors, and metabolites, in whole-body T. castaneum were measured by high-performance liquid chromatography coupled with electrochemical detection (ECD). Tyrosine occurred in the highest concentration, followed by OA, tryptophan, and 3,4-dihydroxymandelic acid. The amount of OA was much higher than that of tyramine (a precursor of OA in the biosynthetic pathway) and of synephrine (N-methyl OA). DA, 5-HT, E, NE, and their related substances occurred in extremely low quantities compared with OA. Insects were stressed by vibrating at 1,10,100, or 1000 Hz, optically under a 24-h light (15 W, 50 Hz) photoperiod, thermally by changing the incubation temperature from an initial value of 30°C, or by starvation, which resulted in dramatic changes of levels of biogenic amines, including OA.
AB - Such stress factors as mechanical (vibration), thermal (unfavorable temperature), optical (light), and starvation reduced the larval growth of the red flour beetle (Tribolium castaneum Herbst). Various biogenic amines, including octopamine (OA), dopamine (DA), serotonin (5-HT), epinephrine (E), norepinephrine (NE), their precursors, and metabolites, in whole-body T. castaneum were measured by high-performance liquid chromatography coupled with electrochemical detection (ECD). Tyrosine occurred in the highest concentration, followed by OA, tryptophan, and 3,4-dihydroxymandelic acid. The amount of OA was much higher than that of tyramine (a precursor of OA in the biosynthetic pathway) and of synephrine (N-methyl OA). DA, 5-HT, E, NE, and their related substances occurred in extremely low quantities compared with OA. Insects were stressed by vibrating at 1,10,100, or 1000 Hz, optically under a 24-h light (15 W, 50 Hz) photoperiod, thermally by changing the incubation temperature from an initial value of 30°C, or by starvation, which resulted in dramatic changes of levels of biogenic amines, including OA.
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U2 - 10.1271/bbb.57.2085
DO - 10.1271/bbb.57.2085
M3 - Article
AN - SCOPUS:85007669646
SN - 0916-8451
VL - 57
SP - 2085
EP - 2089
JO - Bioscience, biotechnology, and biochemistry
JF - Bioscience, biotechnology, and biochemistry
IS - 12
ER -