Stratum intermedium lineage diverges from ameloblast lineage via Notch signaling

Hidemitsu Harada, Yasuo Ichimori, Tamaki Yokohama-Tamaki, Hayato Ohshima, Shintaro Kawano, Ken Ichi Katsube, Satoshi Wakisaka

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)


The stratum intermedium develops as flattened cell layers on the proximal side of the ameloblast layer during tooth development. However, little information is available regarding the origin and the role. In this study, we indicate that some stratum intermedium cells originate from the inner enamel epithelium (IEE) in rat incisor organ cultures using DiI as a tracer. Immunohistochemical and in situ hybridization studies showed that the stratum intermedium cells express the Notch1 protein and Hes1 mRNAs, while the IEE and ameloblasts express the Jagged1. Further, we examined the role of Notch signaling using the dental epithelial cell line HAT-7. Recombinant Jagged1 protein enhanced the appearance of stratum intermedium cells in HAT-7 cultures and neutralization with an anti-Jagged1 antibody inhibited these effects. Additionally, overexpression of the Notch1 internal domain increased the number of stratum intermedium cells. We hypothesize that the stratum intermedium lineage differentiates from the ameloblast lineage via Notch signaling.

Original languageEnglish
Pages (from-to)611-616
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - Feb 10 2006

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Stratum intermedium lineage diverges from ameloblast lineage via Notch signaling'. Together they form a unique fingerprint.

Cite this