TY - GEN
T1 - Stiffness-index map based on single cell-spheroid analysis using robot integrated microfluidic chiip
AU - Ito, Keitaro
AU - Sakuma, Shinya
AU - Kimura, Masaki
AU - Takebe, Takanori
AU - Kaneko, Makoto
AU - Arai, Fumihito
N1 - Publisher Copyright:
© 2016 IEEE.
Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 2016/2/26
Y1 - 2016/2/26
N2 - We present a method which can evaluate stiffness of cell-spheroids, and show the measurement results of the stiffness changes with respect to the culture time. To obtain the stiffness-index map, where the map shows the relationship between the size and stiffness of cell-spheroids, we applied on-chip measurement method using a robot integrated microfluidic chip (robochip). The robochip has a microchannel and a pair of on-chip probes which contains a force sensor. By measuring the stiffness in microfluidic channel, we can achieve a stable and high-throughput evaluation of mechanical characteristics. The stiffness of the single spheroid is evaluated from the reactive force when the target spheroid is deformed by the on-chip probes. The stiffness-index of cell-spheroid is proposed, and the evaluation results of the stiffness index shows that the index increased along the culture days. The proposed system will provide us a new index for the cell-quality evaluation.
AB - We present a method which can evaluate stiffness of cell-spheroids, and show the measurement results of the stiffness changes with respect to the culture time. To obtain the stiffness-index map, where the map shows the relationship between the size and stiffness of cell-spheroids, we applied on-chip measurement method using a robot integrated microfluidic chip (robochip). The robochip has a microchannel and a pair of on-chip probes which contains a force sensor. By measuring the stiffness in microfluidic channel, we can achieve a stable and high-throughput evaluation of mechanical characteristics. The stiffness of the single spheroid is evaluated from the reactive force when the target spheroid is deformed by the on-chip probes. The stiffness-index of cell-spheroid is proposed, and the evaluation results of the stiffness index shows that the index increased along the culture days. The proposed system will provide us a new index for the cell-quality evaluation.
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U2 - 10.1109/MEMSYS.2016.7421582
DO - 10.1109/MEMSYS.2016.7421582
M3 - Conference contribution
AN - SCOPUS:84970965511
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 157
EP - 160
BT - MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016
Y2 - 24 January 2016 through 28 January 2016
ER -