TY - JOUR
T1 - Sterol effect on interaction between amphidinol 3 and liposomal membrane as evidenced by surface plasmon resonance
AU - Swasono, Respati T.
AU - Mouri, Ryota
AU - Morsy, Nagy
AU - Matsumori, Nobuaki
AU - Oishi, Tohru
AU - Murata, Michio
N1 - Funding Information:
This work was supported by Grants-in-Aid for Scientific Research (S) (No. 18101010), for Priority Area (A) (No. 16073211), and for Young Scientists (A) (No. 17681027) from MEXT, Japan as well as by The Naito Foundation. A Monbukagakusho scholarship to R.T.S. is also greatly acknowledged.
Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2010/4/1
Y1 - 2010/4/1
N2 - The affinity of amphidinol 3 (AM3) to phospholipid membranes in the presence and absence of sterol was examined by surface plasmon resonance (SPR) experiments. The results showed that AM3 has 1000 and 5300 times higher affinity for cholesterol- and ergosterol-containing liposomes, respectively, than those without sterol. The two-state reaction model well reproduced the sensor grams, which indicated that the interaction is composed of two steps, which correspond to binding to the membrane and internalization to form stable complexes.
AB - The affinity of amphidinol 3 (AM3) to phospholipid membranes in the presence and absence of sterol was examined by surface plasmon resonance (SPR) experiments. The results showed that AM3 has 1000 and 5300 times higher affinity for cholesterol- and ergosterol-containing liposomes, respectively, than those without sterol. The two-state reaction model well reproduced the sensor grams, which indicated that the interaction is composed of two steps, which correspond to binding to the membrane and internalization to form stable complexes.
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U2 - 10.1016/j.bmcl.2010.02.025
DO - 10.1016/j.bmcl.2010.02.025
M3 - Article
C2 - 20207137
AN - SCOPUS:77949490838
SN - 0960-894X
VL - 20
SP - 2215
EP - 2218
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 7
ER -