TY - JOUR
T1 - Steroid responsive regulation of IFNγ2 alternative splicing and its possible role in germ cell proliferation in medaka
AU - Mohapatra, Sipra
AU - Chakraborty, Tapas
AU - Miyagawa, Shinichi
AU - Zhou, Linyan
AU - Ohta, Kohei
AU - Iguchi, Taisen
AU - Nagahama, Yoshitaka
N1 - Funding Information:
This work was supported in part by Grants-in-Aid for the SORST Research Project of Japan Science and Technology Corporation , Scientific Research from the Ministry of Education, Science and Culture of Japan and JSPS Kakenhi Grant Number 23688022 . The authors are thankful to National Bioresource Project (NBRP)-Medaka for providing the medaka strains. We sincerely thank Prof. Deshou Wang, Southwest University, Chongqing, P. R. China, for the critical reading of the manuscript.
Publisher Copyright:
© 2014 Elsevier Ireland Ltd.
PY - 2015/1/5
Y1 - 2015/1/5
N2 - Interferon gamma (. IFNγ) is an active player in estrogen dependent immuno-regulation of fish. The present work was aimed to characterize the alternatively spliced isoforms of IFNγ2 in the gonadal sex development in medaka. Phylogenetic analysis demonstrated that IFNγ2a and 2b were clustered with fish specific interferon gamma. Our in vitro promoter and mini-genome analysis data confirmed that alternative splicing of IFNγ2 is regulated by estrogens and androgens. Tissue distribution, quantitative PCR and ISH data demonstrated ubiquitous expression of IFNγ2a, while IFNγ2b was only expressed predominantly in female germ cells than males. This was further confirmed by germ cell specific GFP signals in the IFNγ2b-GFP over-expressed embryos and specific induction of IFNγ2b expression in the BrdU positive cells. All together our data suggest that steroid responsive alternatively spliced IFNγ2b isoforms might have some indirect roles in germ cell proliferation and thus can be an important candidate for immuno-reproductive interaction studies.
AB - Interferon gamma (. IFNγ) is an active player in estrogen dependent immuno-regulation of fish. The present work was aimed to characterize the alternatively spliced isoforms of IFNγ2 in the gonadal sex development in medaka. Phylogenetic analysis demonstrated that IFNγ2a and 2b were clustered with fish specific interferon gamma. Our in vitro promoter and mini-genome analysis data confirmed that alternative splicing of IFNγ2 is regulated by estrogens and androgens. Tissue distribution, quantitative PCR and ISH data demonstrated ubiquitous expression of IFNγ2a, while IFNγ2b was only expressed predominantly in female germ cells than males. This was further confirmed by germ cell specific GFP signals in the IFNγ2b-GFP over-expressed embryos and specific induction of IFNγ2b expression in the BrdU positive cells. All together our data suggest that steroid responsive alternatively spliced IFNγ2b isoforms might have some indirect roles in germ cell proliferation and thus can be an important candidate for immuno-reproductive interaction studies.
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U2 - 10.1016/j.mce.2014.10.012
DO - 10.1016/j.mce.2014.10.012
M3 - Article
C2 - 25458697
AN - SCOPUS:84913553460
SN - 0303-7207
VL - 400
SP - 61
EP - 70
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
ER -