TY - JOUR
T1 - Sterilization of microorganisms with supercritical carbon dioxide
AU - Kamihira, Masamichi
AU - Taniguchi, Masayuki
AU - Kobayashi, Takeshi
N1 - Funding Information:
Acknowledgments. The authors wish to thank Messrs. T. Aki, T. Murakami and H. Tashiro of Mitsubishi Kakoki Co. for their helpful advice and kind suggestions. This work was supported in part by a Grant-in-Aid for Special Project Research (No. 59124003) from the Ministry of Education, Science and Culture of Japan.
PY - 1987/2
Y1 - 1987/2
N2 - The sterilizing effect of supercritical carbon dioxide (SC-C02) is described as compared with aseous C02(G-C02) and liquid C02(L-C02). Baker’s yeast, Escherichia coli, Staphylococcus aureus and conidia of Aspergillus niger were sterilized by treating with SC-C02 at 200 atm and 35°C when the water content of each microorganism was 70-90%. However, dry cells with a water content of 2 - 10% could not be sterilized under the same conditions. No sterilizing effect from SC- C02 on endospores of Bacillus subtilis and B. stearothermophilus was observed. G-C02 and L-C02produced no sterilizing effect against both wet and dry baker’s yeast cells, while wet E. coli cells were sterilized with G-C02. Although dry conidia of A. niger could not be sterilized with SC-C02alone, the addition of ethanol or acetic acid to SC-C02 made it possible to sterilize these. Without any decrease in their enzymatic activities, E. preparations were sterilized with SC-C02.
AB - The sterilizing effect of supercritical carbon dioxide (SC-C02) is described as compared with aseous C02(G-C02) and liquid C02(L-C02). Baker’s yeast, Escherichia coli, Staphylococcus aureus and conidia of Aspergillus niger were sterilized by treating with SC-C02 at 200 atm and 35°C when the water content of each microorganism was 70-90%. However, dry cells with a water content of 2 - 10% could not be sterilized under the same conditions. No sterilizing effect from SC- C02 on endospores of Bacillus subtilis and B. stearothermophilus was observed. G-C02 and L-C02produced no sterilizing effect against both wet and dry baker’s yeast cells, while wet E. coli cells were sterilized with G-C02. Although dry conidia of A. niger could not be sterilized with SC-C02alone, the addition of ethanol or acetic acid to SC-C02 made it possible to sterilize these. Without any decrease in their enzymatic activities, E. preparations were sterilized with SC-C02.
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U2 - 10.1080/00021369.1987.10868053
DO - 10.1080/00021369.1987.10868053
M3 - Article
AN - SCOPUS:0001570980
SN - 0002-1369
VL - 51
SP - 407
EP - 412
JO - Agricultural and Biological Chemistry
JF - Agricultural and Biological Chemistry
IS - 2
ER -