TY - JOUR
T1 - Stabilization of alanine substituted p53 protein at Ser15, Thr18, and Ser20 in response to ionizing radiation
AU - Yamauchi, Motohiro
AU - Suzuki, Keiji
AU - Kodama, Seiji
AU - Watanabe, Masami
N1 - Funding Information:
This work was supported in part by a grant for scientific research from the Ministry of Education, Culture, Sports, Science and Technology of Japan and by the Research Project Fund for “Studies on the Molecular Biological Basis for Low-Dose Radiation Effects” from the Japan Atomic Energy Research Institute through a contract with the Nuclear Safety Research Association, and by a grant from Japan Society for the Promotion of Science.
PY - 2004/10/22
Y1 - 2004/10/22
N2 - Phosphorylation of p53 at Ser15, Thr18, and Ser20 has been thought to be important for p53 stabilization in response to ionizing radiation. In the present study, we examined the X-ray-induced stabilization of Ala-substituted p53 protein at Ser15, Thr18, and Ser20, whose gene expression was controlled under an ecdyson-inducible promoter. We found that all single-, double-, or triple-Ala-substituted p53 at Ser15, Yhr18, and Ser20 were accumulated in the nucleus similarly to wild-type p53 after X-irradiation. These results indicate that the phosphorylation of p53 at Ser15, Thr18, and Ser20 is not necessarily needed for p53 stabilization in response to ionizing radiation.
AB - Phosphorylation of p53 at Ser15, Thr18, and Ser20 has been thought to be important for p53 stabilization in response to ionizing radiation. In the present study, we examined the X-ray-induced stabilization of Ala-substituted p53 protein at Ser15, Thr18, and Ser20, whose gene expression was controlled under an ecdyson-inducible promoter. We found that all single-, double-, or triple-Ala-substituted p53 at Ser15, Yhr18, and Ser20 were accumulated in the nucleus similarly to wild-type p53 after X-irradiation. These results indicate that the phosphorylation of p53 at Ser15, Thr18, and Ser20 is not necessarily needed for p53 stabilization in response to ionizing radiation.
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U2 - 10.1016/j.bbrc.2004.08.175
DO - 10.1016/j.bbrc.2004.08.175
M3 - Article
C2 - 15381086
AN - SCOPUS:4544368682
SN - 0006-291X
VL - 323
SP - 906
EP - 911
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -