Stability of mutant actins

D. R. Drummond, E. S. Hennessey, J. C. Sparrow

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Mutants of the Drosophila Act88F actin gene were transcribed and translated in vitro and their relative stabilities were examined using urea gradient gel electrophoresis. Most of the mutant actins (E334K, E364K, G366D, G368E and R372H) were as stable as the wild-type. V339I had a slight decrease in stability, and E316K was the least stable. The causes of the differences are discussed and contrasted with the behaviour of the mutants in vivo, where E316K has normal stability and V339I is the least stable.

Original languageEnglish
Pages (from-to)301-303
Number of pages3
JournalBiochemical Journal
Issue number1
Publication statusPublished - 1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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