Stability of DNA/Td3717 complexes for gene transfection, defined by the size and polydispersity of the complex

Shinichi Kuriyama; Yasushi Taguchi; Kazuto Watanabe; Kanako Nishimura; Kazutoshi Yanagibashi; Yoshiki Katayama; Takuro Niidome

doi:10.1016/j.bmc.2009.09.049

Stability of DNA/Td3717 complexes for gene transfection, defined by the size and polydispersity of the complex

Shinichi Kuriyama, Yasushi Taguchi, Kazuto Watanabe, Kanako Nishimura, Kazutoshi Yanagibashi, Yoshiki Katayama, Takuro Niidome

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The amphiphilic α-helical peptide, Td3717, is a bi-functional synthetic peptide that acts as both a polycation for DNA binding and a ligand for targeted delivery to tumor cells. Td3717 forms a stable complex with plasmid DNA, and the complex maintained high transfection efficiency after storage at 4 °C for six months and after four freeze/thaw cycles. During the storage and freeze/thaw cycling, the particle size of the DNA/Td3717 complex remained less than 100 nm. The size of the complex is an important factor for its internalization into cells via the endocytosis pathway; therefore, the stability of the particles will strongly contribute to high transfection efficiencies after storage and repeated freezing/thawing.

Original language	English
Pages (from-to)	7643-7646
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry
Volume	17
Issue number	22
DOIs	https://doi.org/10.1016/j.bmc.2009.09.049
Publication status	Published - Nov 15 2009

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmc.2009.09.049

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title = "Stability of DNA/Td3717 complexes for gene transfection, defined by the size and polydispersity of the complex",

abstract = "The amphiphilic α-helical peptide, Td3717, is a bi-functional synthetic peptide that acts as both a polycation for DNA binding and a ligand for targeted delivery to tumor cells. Td3717 forms a stable complex with plasmid DNA, and the complex maintained high transfection efficiency after storage at 4 °C for six months and after four freeze/thaw cycles. During the storage and freeze/thaw cycling, the particle size of the DNA/Td3717 complex remained less than 100 nm. The size of the complex is an important factor for its internalization into cells via the endocytosis pathway; therefore, the stability of the particles will strongly contribute to high transfection efficiencies after storage and repeated freezing/thawing.",

author = "Shinichi Kuriyama and Yasushi Taguchi and Kazuto Watanabe and Kanako Nishimura and Kazutoshi Yanagibashi and Yoshiki Katayama and Takuro Niidome",

note = "Funding Information: This research was supported by a Grant-in-Aid for Exploratory Research ( No. 18650124 ) and a Grant-in-Aid for Scientific Research (B) ( No. 19300172 ) from the Japan Society for the Promotion of Science (JSPS) .",

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doi = "10.1016/j.bmc.2009.09.049",

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T1 - Stability of DNA/Td3717 complexes for gene transfection, defined by the size and polydispersity of the complex

AU - Kuriyama, Shinichi

AU - Taguchi, Yasushi

AU - Watanabe, Kazuto

AU - Nishimura, Kanako

AU - Yanagibashi, Kazutoshi

AU - Katayama, Yoshiki

AU - Niidome, Takuro

N1 - Funding Information: This research was supported by a Grant-in-Aid for Exploratory Research ( No. 18650124 ) and a Grant-in-Aid for Scientific Research (B) ( No. 19300172 ) from the Japan Society for the Promotion of Science (JSPS) .

PY - 2009/11/15

Y1 - 2009/11/15

N2 - The amphiphilic α-helical peptide, Td3717, is a bi-functional synthetic peptide that acts as both a polycation for DNA binding and a ligand for targeted delivery to tumor cells. Td3717 forms a stable complex with plasmid DNA, and the complex maintained high transfection efficiency after storage at 4 °C for six months and after four freeze/thaw cycles. During the storage and freeze/thaw cycling, the particle size of the DNA/Td3717 complex remained less than 100 nm. The size of the complex is an important factor for its internalization into cells via the endocytosis pathway; therefore, the stability of the particles will strongly contribute to high transfection efficiencies after storage and repeated freezing/thawing.

AB - The amphiphilic α-helical peptide, Td3717, is a bi-functional synthetic peptide that acts as both a polycation for DNA binding and a ligand for targeted delivery to tumor cells. Td3717 forms a stable complex with plasmid DNA, and the complex maintained high transfection efficiency after storage at 4 °C for six months and after four freeze/thaw cycles. During the storage and freeze/thaw cycling, the particle size of the DNA/Td3717 complex remained less than 100 nm. The size of the complex is an important factor for its internalization into cells via the endocytosis pathway; therefore, the stability of the particles will strongly contribute to high transfection efficiencies after storage and repeated freezing/thawing.

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JO - Bioorganic and Medicinal Chemistry

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ER -

Stability of DNA/Td3717 complexes for gene transfection, defined by the size and polydispersity of the complex

Abstract

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