Sse8647l, a new type II restriction endonuclease from a Streptomyces species cutting at 5'-AG/GWCCT-3'

Yoshizumi Ishino; Yosiko Nomura; Ikunoshin Kato

doi:10.1093/nar/23.5.742

Sse8647l, a new type II restriction endonuclease from a Streptomyces species cutting at 5'-AG/GWCCT-3'

Yoshizumi Ishino
, Yosiko Nomura
, Ikunoshin Kato

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We Isolated and characterized a new type II restriction endonuclease which recognizes the palindromic heptanucleotide sequence 5'-AGGWCCT-3' and cleaves double-stranded DNA after the first G in the sequence from a microorganism belonging to Steptomyces species. This enzyme cleaves adenovirus 2 DNA at eight sites, but does not cleave λ phage, pBR322, pUC18 and 19, M13mp18 and 19, SV40, ColE1 and φX174 DNAs.

Original language	English
Pages (from-to)	742-744
Number of pages	3
Journal	Nucleic acids research
Volume	23
Issue number	5
DOIs	https://doi.org/10.1093/nar/23.5.742
Publication status	Published - Mar 11 1995
Externally published	Yes

All Science Journal Classification (ASJC) codes

Genetics

Access to Document

10.1093/nar/23.5.742

Cite this

@article{88769396ff4a4b5d949ebaf7ce7a7391,

title = "Sse8647l, a new type II restriction endonuclease from a Streptomyces species cutting at 5'-AG/GWCCT-3'",

abstract = "We Isolated and characterized a new type II restriction endonuclease which recognizes the palindromic heptanucleotide sequence 5'-AGGWCCT-3' and cleaves double-stranded DNA after the first G in the sequence from a microorganism belonging to Steptomyces species. This enzyme cleaves adenovirus 2 DNA at eight sites, but does not cleave λ phage, pBR322, pUC18 and 19, M13mp18 and 19, SV40, ColE1 and φX174 DNAs.",

author = "Yoshizumi Ishino and Yosiko Nomura and Ikunoshin Kato",

year = "1995",

month = mar,

day = "11",

doi = "10.1093/nar/23.5.742",

language = "English",

volume = "23",

pages = "742--744",

journal = "Nucleic acids research",

issn = "0305-1048",

publisher = "Oxford University Press",

number = "5",

}

TY - JOUR

T1 - Sse8647l, a new type II restriction endonuclease from a Streptomyces species cutting at 5'-AG/GWCCT-3'

AU - Ishino, Yoshizumi

AU - Nomura, Yosiko

AU - Kato, Ikunoshin

PY - 1995/3/11

Y1 - 1995/3/11

N2 - We Isolated and characterized a new type II restriction endonuclease which recognizes the palindromic heptanucleotide sequence 5'-AGGWCCT-3' and cleaves double-stranded DNA after the first G in the sequence from a microorganism belonging to Steptomyces species. This enzyme cleaves adenovirus 2 DNA at eight sites, but does not cleave λ phage, pBR322, pUC18 and 19, M13mp18 and 19, SV40, ColE1 and φX174 DNAs.

AB - We Isolated and characterized a new type II restriction endonuclease which recognizes the palindromic heptanucleotide sequence 5'-AGGWCCT-3' and cleaves double-stranded DNA after the first G in the sequence from a microorganism belonging to Steptomyces species. This enzyme cleaves adenovirus 2 DNA at eight sites, but does not cleave λ phage, pBR322, pUC18 and 19, M13mp18 and 19, SV40, ColE1 and φX174 DNAs.

UR - https://www.scopus.com/pages/publications/0028986815

UR - https://www.scopus.com/pages/publications/0028986815#tab=citedBy

U2 - 10.1093/nar/23.5.742

DO - 10.1093/nar/23.5.742

M3 - Article

C2 - 7708487

AN - SCOPUS:0028986815

SN - 0305-1048

VL - 23

SP - 742

EP - 744

JO - Nucleic acids research

JF - Nucleic acids research

IS - 5

ER -

Sse8647l, a new type II restriction endonuclease from a Streptomyces species cutting at 5'-AG/GWCCT-3'

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this