TY - GEN
T1 - Sprouting from on-chip vascular networks by a pressure-driven flow
AU - Nakayama, Masamune
AU - Nashimoto, Yuji
AU - Kunita, Itsuki
AU - Nakamasu, Akiko
AU - Torisawa, Yu Suke
AU - Shintaku, Hirofumi
AU - Kotera, Hidetoshi
AU - Nishiyama, Koichi
AU - Miura, Takashi
AU - Yokokawa, Ryuji
PY - 2016
Y1 - 2016
N2 - We quantitatively evaluated how vascular networks reconstructed in a microfluidic device were remodeled under a pressure-driven flow. We fabricated a PDMS device, and co-cultured human umbilical vein endothelial cells (HUVECs) and normal human lung fibroblasts (hLFs) to spontaneously reconstruct vascular networks. After a pressure-driven flow (0-120 μL/h) was applied to the vascular networks for 24 hours, we found the number of angiogenic sprouting increasing with the increase of flow rate and perfusion time.
AB - We quantitatively evaluated how vascular networks reconstructed in a microfluidic device were remodeled under a pressure-driven flow. We fabricated a PDMS device, and co-cultured human umbilical vein endothelial cells (HUVECs) and normal human lung fibroblasts (hLFs) to spontaneously reconstruct vascular networks. After a pressure-driven flow (0-120 μL/h) was applied to the vascular networks for 24 hours, we found the number of angiogenic sprouting increasing with the increase of flow rate and perfusion time.
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M3 - Conference contribution
AN - SCOPUS:85014143921
T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
SP - 429
EP - 430
BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PB - Chemical and Biological Microsystems Society
T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Y2 - 9 October 2016 through 13 October 2016
ER -