Spermidine resets circadian clock phase in nih3t3 cells

Dan Yang, Hideaki Oike, Mitsuhiro Furuse, Shinobu Yasuo

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Irregular light-dark cycles desynchronize the circadian clock via hormonal and neuronal pathways and increase the risk of various diseases. This study demonstrated that a single pulse of spermi-dine—a polyamine—strongly induced circadian phase advances in the presence or absence of dexamethasone (a synthetic glucocorticoid) in NIH3T3 cells transfected with the Bmal1 promo-tor-driven luciferase reporter gene. The spermidine-induced phase advances were 2–3 fold greater than were the dexamethasone-induced shifts. The phase resetting effect of spermidine occurred in a dose-and time-dependent manner and was not blocked by RU486, an antagonist of glucocorti-coid receptors. Spermidine treatment modulated the expression of clock genes within 60 min, which was sooner than changes in the expression of autophagy-related genes. These findings sug-gested that spermidine is a potent modulator of the circadian phase, acting through glucocorticoid receptor-independent pathways, and may be useful for treating diseases related to circadian desyn-chrony.

Original languageEnglish
Pages (from-to)221-227
Number of pages7
JournalBiomedical Research (Japan)
Issue number5
Publication statusPublished - 2021

All Science Journal Classification (ASJC) codes

  • General Biochemistry,Genetics and Molecular Biology


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