Spectral assignments and anisotropy data of cellulose I_?α: ¹³C-NMR chemical shift data of cellulose I_α determined by INADEQUATE and RAI techniques applied to uniformly ¹³C-labeled bacterial celluloses of different Gluconacetobacter xylinus strains

Solid-state ¹³C-NMR spectroscopy was used to characterize native cellulose pellicles from two strains of Gluconacetobacter xylinus (ATCC 53582, ATCC 23769), which had been statically cultivated in Hestrin-Schramm (HS) medium containing fully ¹³C-labeled β-D-glucose-U-¹³C ₆ as the sole source of carbon. For both samples, the ¹³C-NMR chemical shifts were completely assigned for each ¹³C-labeled site of cellulose I_α with the aid of 2D refocused INADEQUATE NMR. To determine the principal chemical shift tensor components, a pulse sequence based on the recoupling of anisotropy information (RAI) was applied at 10 kHz MAS. The detailed ¹³C tensors of cellulose I_α from different bacterial celluloses are thus available now for the first time, and these results have been compared with previously published data of nonenriched material and with theoretical predictions.