TY - GEN
T1 - Specific detection of Escherichia coli by using metallic mesh sensor in THz region
AU - Kurita, Ippei
AU - Suzuki, Tetsuhito
AU - Kondo, Naoshi
AU - Kondo, Takashi
AU - Kamba, Seiji
AU - Miura, Yoshiko
AU - Ogawa, Yuichi
N1 - Publisher Copyright:
© 2014 IEEE.
PY - 2014/10/17
Y1 - 2014/10/17
N2 - We demonstrated specific and rapid detection of Escherichia coli (E. coli) without culturing and labeling, using a metallic mesh sensor. Streptavidin and biotinylated antibody, specific for E. coli, were immobilized on the metallic mesh surface via amination and maleimidation. E. coli in PBS were captured, and attached to the surface of the metallic mesh, by immunoreaction with the antibody for E. coli. Transmission spectra of these samples were then measured around 10 THz, where the size of the generated, localized, electric field is the same as that of E. coli. After demonstrating a significant relationship between the frequency shift on the metallic mesh sensor and antibody for E. coli concentration, we then went on to specifically and quantitatively measure E. coli concentration. As E. coli concentration increased so did the spectral frequency shift, with detection limit being observed at 106 mL-1.
AB - We demonstrated specific and rapid detection of Escherichia coli (E. coli) without culturing and labeling, using a metallic mesh sensor. Streptavidin and biotinylated antibody, specific for E. coli, were immobilized on the metallic mesh surface via amination and maleimidation. E. coli in PBS were captured, and attached to the surface of the metallic mesh, by immunoreaction with the antibody for E. coli. Transmission spectra of these samples were then measured around 10 THz, where the size of the generated, localized, electric field is the same as that of E. coli. After demonstrating a significant relationship between the frequency shift on the metallic mesh sensor and antibody for E. coli concentration, we then went on to specifically and quantitatively measure E. coli concentration. As E. coli concentration increased so did the spectral frequency shift, with detection limit being observed at 106 mL-1.
UR - http://www.scopus.com/inward/record.url?scp=84919741127&partnerID=8YFLogxK
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U2 - 10.1109/URSIGASS.2014.6930066
DO - 10.1109/URSIGASS.2014.6930066
M3 - Conference contribution
AN - SCOPUS:84919741127
T3 - 2014 31th URSI General Assembly and Scientific Symposium, URSI GASS 2014
BT - 2014 31th URSI General Assembly and Scientific Symposium, URSI GASS 2014
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 31st General Assembly and Scientific Symposium of the International Union of Radio Science, URSI GASS 2014
Y2 - 16 August 2014 through 23 August 2014
ER -