Somatic mutations of epidermal growth factor receptor in colorectal carcinoma

Hisashi Nagahara, Koshi Mimori, Mitsuhiko Ohta, Tohru Utsunomiya, Hiroshi Inoue, Graham F. Barnard, Masaichi Ohira, Kosei Hirakawa, Masaki Mori

Research output: Contribution to journalArticlepeer-review

123 Citations (Scopus)


Purpose: Somatic mutations of the epidermal growth factor receptor (EGFR) gene may predict the sensitivity of non-small cell lung carcinoma to gefitinib. However, no mutations have been reported for colorectal carcinoma. We therefore analyzed EGFR mutations in colorectal adenocarcinomas by the combined use of laser microdissection and sequencing of genomic DNA. Experimental Design: We examined 11 representative colorectal adenocarcinoma cell lines and 33 clinical samples of colorectal carcinoma. In the clinical cases, we carefully dissected only carcinoma cells from frozen sections by laser microdissection. After DNA extraction and PCR, we examined EGFR mutations by sequencing genomic DNA. Results: None of 11 colorectal carcinoma cell lines exhibited somatic mutations, but 4 of 33 clinical tumors (12%) exhibited mutations in the EGFR kinase domain. This may be the first report of somatic mutations in colorectal adenocarcinoma. Conclusions: Our findings suggest that a distinct minority of colorectal adenocarcinomas exhibit somatic mutations of EGFR, and these tumors may be susceptible to gefitinib treatment.

Original languageEnglish
Pages (from-to)1368-1371
Number of pages4
JournalClinical Cancer Research
Issue number4
Publication statusPublished - Feb 15 2005

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research


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