TY - GEN
T1 - Single DNA/protein interaction analysis using quantum dot conjugates and microfluidic systems
AU - Onoshima, D.
AU - Kaji, N.
AU - Tokeshi, M.
AU - Baba, Y.
N1 - Publisher Copyright:
© 2007 CBMS.
PY - 2007
Y1 - 2007
N2 - We have designed a microfluidic detection platform in which we can monitor and analyze single DNA/protein dynamic interaction using “single-molecule fluorescence tracing”. The use of quantum dot (QD) labelling on DNA, polydimethylsiloxane (PDMS) microchip, micro patterning of active enzymes, and total internal reflection fluorescence (TIRF) microscope was successfully applied for nano-space experiments in micrometer-sized channels. We used this detection platform to observe the enzymatic reaction of restriction endonuclease (EcoRI) and measured the duration of its cleavage reaction.
AB - We have designed a microfluidic detection platform in which we can monitor and analyze single DNA/protein dynamic interaction using “single-molecule fluorescence tracing”. The use of quantum dot (QD) labelling on DNA, polydimethylsiloxane (PDMS) microchip, micro patterning of active enzymes, and total internal reflection fluorescence (TIRF) microscope was successfully applied for nano-space experiments in micrometer-sized channels. We used this detection platform to observe the enzymatic reaction of restriction endonuclease (EcoRI) and measured the duration of its cleavage reaction.
UR - http://www.scopus.com/inward/record.url?scp=85088681716&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85088681716&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85088681716
T3 - Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
SP - 1619
EP - 1621
BT - Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
A2 - Viovy, Jean-Louis
A2 - Tabeling, Patrick
A2 - Descroix, Stephanie
A2 - Malaquin, Laurent
PB - Chemical and Biological Microsystems Society
T2 - 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
Y2 - 7 October 2007 through 11 October 2007
ER -