Single-chain Fv antibody stimulates biosynthesis of secondary metabolites in plants

Waraporn Putalun, Hiroyuki Tanaka, Yukihiro Shoyama

Research output: Chapter in Book/Report/Conference proceedingChapter


A recombinant antibody fragment-single chain-fragment-variable (scFv) antibody can control the concentration of solasodine glycosides in hairy root cultures of Solanum khasianum transformed by the anti-solamargine (As)-scFv gene. The characterization of the As-scFv protein expressed in Escherichia coli was identical to those of the parent monoclonal antibody (MAb). Up to 220 ng recombinant As-scFv was expressed per milligram of soluble protein in transgenic hairy root cultures of S. khasianum. The concentration of solasodine glycosides was 2.3-fold higher in the transgenic than in the wild-type hairy root reflecting by the soluble As-scFv level and antigen binding activities. These phenomena suggested that the scFv antibody expressed in transgenic hairy roots controlled the antigen level representing a novel plant breeding methodology that can produce secondary metabolites, recommending that the method can be called as a missile-type breeding.

Original languageEnglish
Title of host publicationApplications of Plant Metabolic Engineering
PublisherSpringer Netherlands
Number of pages13
ISBN (Electronic)9781402060311
ISBN (Print)9781402060304
Publication statusPublished - 2007

All Science Journal Classification (ASJC) codes

  • General Agricultural and Biological Sciences


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