TY - JOUR
T1 - Simultaneous two-dimensional HPLC determination of free d-serine and d-alanine in the brain and periphery of mutant rats lacking d-amino-acid oxidase
AU - Miyoshi, Yurika
AU - Hamase, Kenji
AU - Okamura, Tadashi
AU - Konno, Ryuichi
AU - Kasai, Noriyuki
AU - Tojo, Yosuke
AU - Zaitsu, Kiyoshi
N1 - Funding Information:
This work was supported in part by the Grants from New Energy and Industrial Technology Development Organization (NEDO) of Japan . The authors appreciate Shiseido Co., Ltd. (Tokyo, Japan), for technical support.
PY - 2011/11/1
Y1 - 2011/11/1
N2 - A fully automated two-dimensional HPLC system combining a microbore-ODS column and a narrowbore-enantioselective column was designed and validated, and the amounts of d-serine (d-Ser) and d-alanine (d-Ala) in various tissues and physiological fluids of Long-Evans agouti/SENDAI (LEA/Sen) rats lacking d-amino-acid oxidase (DAO) were determined. Intra- and inter-day precision was less than 4.3% and accuracy ranged between 99.9 and 104%. LEA/Sen rats were reported to lack DAO in their kidneys and expected to be a novel mutant animal lacking DAO, however, the amounts of d-amino acids in the LEA/Sen rats have not been investigated. In the present study, the intrinsic amounts of d-Ser and d-Ala, which are neuromodulators of the N-methyl-d-aspartate (NMDA) receptors, were determined in seven brain tissues, four peripheral tissues, plasma and urine of the LEA/Sen rats and compared to those of the control (Wistar and SD) rats having normal DAO activity. The levels of d-Ser in the tissues and physiological fluids of the LEA/Sen rats were significantly higher than those of the Wistar and SD rats except for the frontal brain regions. Concerning d-Ala, the amounts in the tissues and physiological fluids of the LEA/Sen rats were drastically increased compared to those of the Wistar and SD rats. These results indicate that the intrinsic amounts of d-Ser and d-Ala in the tissues of rats are regulated by DAO, and that LEA/Sen rats would be useful for the study of NMDA receptor-related diseases in which DAO is implicated.
AB - A fully automated two-dimensional HPLC system combining a microbore-ODS column and a narrowbore-enantioselective column was designed and validated, and the amounts of d-serine (d-Ser) and d-alanine (d-Ala) in various tissues and physiological fluids of Long-Evans agouti/SENDAI (LEA/Sen) rats lacking d-amino-acid oxidase (DAO) were determined. Intra- and inter-day precision was less than 4.3% and accuracy ranged between 99.9 and 104%. LEA/Sen rats were reported to lack DAO in their kidneys and expected to be a novel mutant animal lacking DAO, however, the amounts of d-amino acids in the LEA/Sen rats have not been investigated. In the present study, the intrinsic amounts of d-Ser and d-Ala, which are neuromodulators of the N-methyl-d-aspartate (NMDA) receptors, were determined in seven brain tissues, four peripheral tissues, plasma and urine of the LEA/Sen rats and compared to those of the control (Wistar and SD) rats having normal DAO activity. The levels of d-Ser in the tissues and physiological fluids of the LEA/Sen rats were significantly higher than those of the Wistar and SD rats except for the frontal brain regions. Concerning d-Ala, the amounts in the tissues and physiological fluids of the LEA/Sen rats were drastically increased compared to those of the Wistar and SD rats. These results indicate that the intrinsic amounts of d-Ser and d-Ala in the tissues of rats are regulated by DAO, and that LEA/Sen rats would be useful for the study of NMDA receptor-related diseases in which DAO is implicated.
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U2 - 10.1016/j.jchromb.2010.08.024
DO - 10.1016/j.jchromb.2010.08.024
M3 - Article
C2 - 20851062
AN - SCOPUS:82555189372
SN - 1570-0232
VL - 879
SP - 3184
EP - 3189
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 29
ER -