TY - GEN
T1 - Simultaneous separation, metering and dilution of plasma from human whole blood using a microchip with an interchannel microstructure
AU - Tachi, Tomoya
AU - Kaji, Noritada
AU - Okamoto, Yukihiro
AU - Tokeshi, Manabu
AU - Baba, Yoshinobu
PY - 2009
Y1 - 2009
N2 - In this research, we designed and operated a microchip with an interchannel microstructure for separation of plasma from human whole blood and for metering and diluting the plasma. The plasma separation was based on both cross-flow filtration and sedimentation of red blood cells in the microchannels. Metering and diluting the plasma was based on volume control of liquid in the microchannels by syringe pumps. Simultaneous operations to separate, meter and dilute plasma obtained from whole blood were done in microchannels using this microchip, and plasma precisely diluted by a factor of 6 was generated without hemolysis in the microchannels in about 3 minutes. This microchip is easily connected with other microchannels and microfluidic devices for quantitative analyses.
AB - In this research, we designed and operated a microchip with an interchannel microstructure for separation of plasma from human whole blood and for metering and diluting the plasma. The plasma separation was based on both cross-flow filtration and sedimentation of red blood cells in the microchannels. Metering and diluting the plasma was based on volume control of liquid in the microchannels by syringe pumps. Simultaneous operations to separate, meter and dilute plasma obtained from whole blood were done in microchannels using this microchip, and plasma precisely diluted by a factor of 6 was generated without hemolysis in the microchannels in about 3 minutes. This microchip is easily connected with other microchannels and microfluidic devices for quantitative analyses.
UR - http://www.scopus.com/inward/record.url?scp=84901744753&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901744753&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901744753
SN - 9780979806421
T3 - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 427
EP - 429
BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Y2 - 1 November 2009 through 5 November 2009
ER -