Simultaneous profiling of 17 steroid hormones for the evaluation of endocrine-disrupting chemicals in H295R cells

Udi Jumhawan; Toshiyuki Yamashita; Kazuya Ishida; Eiichiro Fukusaki; Takeshi Bamba

doi:10.4155/bio-2016-0149

Simultaneous profiling of 17 steroid hormones for the evaluation of endocrine-disrupting chemicals in H295R cells

Udi Jumhawan, Toshiyuki Yamashita, Kazuya Ishida, Eiichiro Fukusaki, Takeshi Bamba

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Aim: There is urgent need to develop a new protocol for the evaluation of chemical substances to potentially interact with the endocrine system and induce numerous pathological issues. The recently validated in vitro screening assay is limited on monitoring two steroid hormones. Methodology & results: The H295R model cell was exposed to seven endocrine disrupting chemicals (EDCs). The levels of 17 steroid hormones in cell extracts were subsequently determined by a quantitative targeted GC/MS/MS method. Through wide coverage, this system managed to capture the effects of exposure to increasing EDCs concentrations in the entire steroidogenic pathways. Conclusion: The developed approach could be beneficial for the mechanistic investigation of EDCs.

Original language	English
Pages (from-to)	67-79
Number of pages	13
Journal	Bioanalysis
Volume	9
Issue number	1
DOIs	https://doi.org/10.4155/bio-2016-0149
Publication status	Published - Jan 2017

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Pharmacology, Toxicology and Pharmaceutics(all)
Clinical Biochemistry
Medical Laboratory Technology

Access to Document

10.4155/bio-2016-0149

Cite this

@article{137d5491c4174db5801ae1985f5d4db0,

title = "Simultaneous profiling of 17 steroid hormones for the evaluation of endocrine-disrupting chemicals in H295R cells",

abstract = "Aim: There is urgent need to develop a new protocol for the evaluation of chemical substances to potentially interact with the endocrine system and induce numerous pathological issues. The recently validated in vitro screening assay is limited on monitoring two steroid hormones. Methodology & results: The H295R model cell was exposed to seven endocrine disrupting chemicals (EDCs). The levels of 17 steroid hormones in cell extracts were subsequently determined by a quantitative targeted GC/MS/MS method. Through wide coverage, this system managed to capture the effects of exposure to increasing EDCs concentrations in the entire steroidogenic pathways. Conclusion: The developed approach could be beneficial for the mechanistic investigation of EDCs.",

author = "Udi Jumhawan and Toshiyuki Yamashita and Kazuya Ishida and Eiichiro Fukusaki and Takeshi Bamba",

year = "2017",

month = jan,

doi = "10.4155/bio-2016-0149",

language = "English",

volume = "9",

pages = "67--79",

journal = "Bioanalysis",

issn = "1757-6180",

publisher = "Future Science",

number = "1",

}

TY - JOUR

T1 - Simultaneous profiling of 17 steroid hormones for the evaluation of endocrine-disrupting chemicals in H295R cells

AU - Jumhawan, Udi

AU - Yamashita, Toshiyuki

AU - Ishida, Kazuya

AU - Fukusaki, Eiichiro

AU - Bamba, Takeshi

PY - 2017/1

Y1 - 2017/1

N2 - Aim: There is urgent need to develop a new protocol for the evaluation of chemical substances to potentially interact with the endocrine system and induce numerous pathological issues. The recently validated in vitro screening assay is limited on monitoring two steroid hormones. Methodology & results: The H295R model cell was exposed to seven endocrine disrupting chemicals (EDCs). The levels of 17 steroid hormones in cell extracts were subsequently determined by a quantitative targeted GC/MS/MS method. Through wide coverage, this system managed to capture the effects of exposure to increasing EDCs concentrations in the entire steroidogenic pathways. Conclusion: The developed approach could be beneficial for the mechanistic investigation of EDCs.

AB - Aim: There is urgent need to develop a new protocol for the evaluation of chemical substances to potentially interact with the endocrine system and induce numerous pathological issues. The recently validated in vitro screening assay is limited on monitoring two steroid hormones. Methodology & results: The H295R model cell was exposed to seven endocrine disrupting chemicals (EDCs). The levels of 17 steroid hormones in cell extracts were subsequently determined by a quantitative targeted GC/MS/MS method. Through wide coverage, this system managed to capture the effects of exposure to increasing EDCs concentrations in the entire steroidogenic pathways. Conclusion: The developed approach could be beneficial for the mechanistic investigation of EDCs.

UR - http://www.scopus.com/inward/record.url?scp=85002958236&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85002958236&partnerID=8YFLogxK

U2 - 10.4155/bio-2016-0149

DO - 10.4155/bio-2016-0149

M3 - Article

C2 - 27921452

AN - SCOPUS:85002958236

SN - 1757-6180

VL - 9

SP - 67

EP - 79

JO - Bioanalysis

JF - Bioanalysis

IS - 1

ER -

Simultaneous profiling of 17 steroid hormones for the evaluation of endocrine-disrupting chemicals in H295R cells

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this