TY - JOUR
T1 - Simultaneous determination of paraquat and diquat in human tissues by high-performance liquid chromatography
AU - Ito, Shinji
AU - Nagata, Takeaki
AU - Kudo, Keiko
AU - Kimura, Kojiro
AU - Imamura, Tohru
N1 - Funding Information:
We thank ICI Japan Ltd. for providing PQ and DQ, and M. Ohara for helpful comments. This work was supportedi n part by a Grant-in-Aid for Scientific Research (No. 04 8070 42) from the JapaneseM inistery of Education.
PY - 1993/7/23
Y1 - 1993/7/23
N2 - A simple, sensitive, reliable, and economical method for simultaneous determination of paraquat dichloride and diquat dibromide in human biological materials has been developed, using high-performance liquid chromatography. The drugs were extracted from the sample with a Sep-Pak C18 cartridge and applied to a chromatograph with the internal standard, l-tyrosine. Paraquat and diquat were clearly separated on the octadecylsilica column with a mobile phase of 0.5% potassium bromide in 5% methanol solution, containing triethylamine (1 ml/l). The pH of the mobile phase was adjusted to 3-4 with 1.3 M phosphoric acid. Two ultraviolet wavelengths were selected, 256 nm for paraquat as well as the internal standard, and 310 nm for diquat. The calibration curves were linear in the concentration range 0.1-10 μg/g, and the lower limit of detection was 0.05 μg/g. We used this method to examine the concentrations of paraquat and diquat in tissues of an individual at autopsy.
AB - A simple, sensitive, reliable, and economical method for simultaneous determination of paraquat dichloride and diquat dibromide in human biological materials has been developed, using high-performance liquid chromatography. The drugs were extracted from the sample with a Sep-Pak C18 cartridge and applied to a chromatograph with the internal standard, l-tyrosine. Paraquat and diquat were clearly separated on the octadecylsilica column with a mobile phase of 0.5% potassium bromide in 5% methanol solution, containing triethylamine (1 ml/l). The pH of the mobile phase was adjusted to 3-4 with 1.3 M phosphoric acid. Two ultraviolet wavelengths were selected, 256 nm for paraquat as well as the internal standard, and 310 nm for diquat. The calibration curves were linear in the concentration range 0.1-10 μg/g, and the lower limit of detection was 0.05 μg/g. We used this method to examine the concentrations of paraquat and diquat in tissues of an individual at autopsy.
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U2 - 10.1016/0378-4347(93)80429-8
DO - 10.1016/0378-4347(93)80429-8
M3 - Article
C2 - 8376524
AN - SCOPUS:0027290911
SN - 0378-4347
VL - 617
SP - 119
EP - 123
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - 1
ER -