TY - JOUR
T1 - Simultaneous determination of biogenic amines, their precursors and metabolites in a single brain of the cricket using high-performance liquid chromatography with amperometric detection
AU - Nagao, Takashi
AU - Tanimura, Teiichi
N1 - Funding Information:
discussion and technical assistance, and Dr. P.L. Newland for critically reading the manuscript. This study was made possible through the kind loan of the HPLC-ED system from Shimadzu Corporation. We also thank Miss M. Wa-tanabe for her expert technical assistance. A part of this study was done as the Co-operative Research Project No. 59157 of the National Institute for Basic Biology.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1989
Y1 - 1989
N2 - An analytical procedure has been developed for the simultaneous determination of biogenic amines, their precursors and metabolites by high-performance liquid chromatography with amperometric electrochemical detection. Following careful adjustment of various factors involved in the separation efficiency, reversed-phase chromatography with an ion-pairing technique gave simultaneous separation of nineteen biogenic amines and related substances. Peak identification was confirmed by comparison with hydrodynamic voltammograms. The method was sensitive enough to detect each substance in the picomole range. The procedure was applied to quantitate the amount of biogenic amines in a single brain of the cricket.
AB - An analytical procedure has been developed for the simultaneous determination of biogenic amines, their precursors and metabolites by high-performance liquid chromatography with amperometric electrochemical detection. Following careful adjustment of various factors involved in the separation efficiency, reversed-phase chromatography with an ion-pairing technique gave simultaneous separation of nineteen biogenic amines and related substances. Peak identification was confirmed by comparison with hydrodynamic voltammograms. The method was sensitive enough to detect each substance in the picomole range. The procedure was applied to quantitate the amount of biogenic amines in a single brain of the cricket.
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U2 - 10.1016/S0378-4347(00)82551-7
DO - 10.1016/S0378-4347(00)82551-7
M3 - Article
C2 - 2592516
AN - SCOPUS:0024455461
SN - 0378-4347
VL - 496
SP - 39
EP - 53
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - C
ER -