Simple assay for antitumour immunoactive glycoprotein derived from Chlorella vulgaris strain CK22 using eLISA

Kiyoshi Noda; Kuniaki Tanaka; Akira Yamada; Jyunko Ogata; Hiroyuki Tanaka; Yukihiro Shoyama

doi:10.1002/ptr.1021

Simple assay for antitumour immunoactive glycoprotein derived from Chlorella vulgaris strain CK22 using eLISA

Kiyoshi Noda, Kuniaki Tanaka, Akira Yamada, Jyunko Ogata, Hiroyuki Tanaka, Yukihiro Shoyama

Pharmaceutical Health Care and Sciences

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

A quantitative ELISA system was developed using a monoclonal antibody (MAb) specific for an antitumour immunoactive glycoprotein (CVS) derived from C. vulgaris strain CK22. The full measuring range of the assay extends from 0.63 to 10.0 ng/mL of CVS. Although no cross-reaction was observed to proteins tested or other biological response modifiers (BRMs) derived from different sources, cross-reactions were found with culture supernatants from two other strains of C. vulgaris having a strong antitumour immunoactivity. Treatment of CVS with protease, acid or alkali weakened or completely eliminated the reactivity against the MAb and also its antitumour immunoactivities. This ELISA system is suitable for the biologically active form of CVS derived from C. vulgaris strain CK22 and related immunoactive strains.

Original language	English
Pages (from-to)	581-585
Number of pages	5
Journal	Phytotherapy Research
Volume	16
Issue number	6
DOIs	https://doi.org/10.1002/ptr.1021
Publication status	Published - Sept 2002

All Science Journal Classification (ASJC) codes

Pharmacology

Access to Document

10.1002/ptr.1021

Cite this

@article{02b4114e2a5a4018922dc66e9e265505,

title = "Simple assay for antitumour immunoactive glycoprotein derived from Chlorella vulgaris strain CK22 using eLISA",

abstract = "A quantitative ELISA system was developed using a monoclonal antibody (MAb) specific for an antitumour immunoactive glycoprotein (CVS) derived from C. vulgaris strain CK22. The full measuring range of the assay extends from 0.63 to 10.0 ng/mL of CVS. Although no cross-reaction was observed to proteins tested or other biological response modifiers (BRMs) derived from different sources, cross-reactions were found with culture supernatants from two other strains of C. vulgaris having a strong antitumour immunoactivity. Treatment of CVS with protease, acid or alkali weakened or completely eliminated the reactivity against the MAb and also its antitumour immunoactivities. This ELISA system is suitable for the biologically active form of CVS derived from C. vulgaris strain CK22 and related immunoactive strains.",

author = "Kiyoshi Noda and Kuniaki Tanaka and Akira Yamada and Jyunko Ogata and Hiroyuki Tanaka and Yukihiro Shoyama",

year = "2002",

month = sep,

doi = "10.1002/ptr.1021",

language = "English",

volume = "16",

pages = "581--585",

journal = "Phytotherapy Research",

issn = "0951-418X",

publisher = "John Wiley and Sons Ltd",

number = "6",

}

TY - JOUR

T1 - Simple assay for antitumour immunoactive glycoprotein derived from Chlorella vulgaris strain CK22 using eLISA

AU - Noda, Kiyoshi

AU - Tanaka, Kuniaki

AU - Yamada, Akira

AU - Ogata, Jyunko

AU - Tanaka, Hiroyuki

AU - Shoyama, Yukihiro

PY - 2002/9

Y1 - 2002/9

N2 - A quantitative ELISA system was developed using a monoclonal antibody (MAb) specific for an antitumour immunoactive glycoprotein (CVS) derived from C. vulgaris strain CK22. The full measuring range of the assay extends from 0.63 to 10.0 ng/mL of CVS. Although no cross-reaction was observed to proteins tested or other biological response modifiers (BRMs) derived from different sources, cross-reactions were found with culture supernatants from two other strains of C. vulgaris having a strong antitumour immunoactivity. Treatment of CVS with protease, acid or alkali weakened or completely eliminated the reactivity against the MAb and also its antitumour immunoactivities. This ELISA system is suitable for the biologically active form of CVS derived from C. vulgaris strain CK22 and related immunoactive strains.

AB - A quantitative ELISA system was developed using a monoclonal antibody (MAb) specific for an antitumour immunoactive glycoprotein (CVS) derived from C. vulgaris strain CK22. The full measuring range of the assay extends from 0.63 to 10.0 ng/mL of CVS. Although no cross-reaction was observed to proteins tested or other biological response modifiers (BRMs) derived from different sources, cross-reactions were found with culture supernatants from two other strains of C. vulgaris having a strong antitumour immunoactivity. Treatment of CVS with protease, acid or alkali weakened or completely eliminated the reactivity against the MAb and also its antitumour immunoactivities. This ELISA system is suitable for the biologically active form of CVS derived from C. vulgaris strain CK22 and related immunoactive strains.

UR - http://www.scopus.com/inward/record.url?scp=0036741522&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036741522&partnerID=8YFLogxK

U2 - 10.1002/ptr.1021

DO - 10.1002/ptr.1021

M3 - Article

C2 - 12237820

AN - SCOPUS:0036741522

SN - 0951-418X

VL - 16

SP - 581

EP - 585

JO - Phytotherapy Research

JF - Phytotherapy Research

IS - 6

ER -

Simple assay for antitumour immunoactive glycoprotein derived from Chlorella vulgaris strain CK22 using eLISA

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this