TY - JOUR
T1 - Simple and sensitive determination of free and total morphine in human liver and kidney using gas chromatography-mass spectrometry
AU - Kudo, Keiko
AU - Ishida, Tomomi
AU - Nishida, Naoki
AU - Yoshioka, Naofumi
AU - Inoue, Hiromasa
AU - Tsuji, Akiko
AU - Ikeda, Noriaki
N1 - Funding Information:
This work was supported by the Research Foundation for Safe Society and Grant-in-Aid for Scientific Research (No. 16390194) from Ministry of Education, Science, Technology, Sports and Culture. We thank M. Ohara (Fukuoka, Japan) for language assistance.
PY - 2006/1/18
Y1 - 2006/1/18
N2 - We developed a reliable, simple and sensitive method to determine free and total morphine in human liver and kidney, using gas chromatography-mass spectrometry (GC-MS). Free morphine or total morphine obtained by acid hydrolysis from 0.2 g tissue sample was extracted using an Extrelut® NT column with an internal standard, dihydrocodeine, followed by trimethylsilylation. The derivatized extract was submitted to GC-MS analysis of EI-SIM mode. The calibration curves of morphine in both liver and kidney samples were linear in the concentration range from 0.005 to 5 μg/g. The lower limits of detection of morphine were 0.005 μg/g. This method proved successful when we determined free and total morphine in liver and kidney obtained from an autopsied man who was mis-ingested morphine compound in the hospital, which resulted in the cause of death being morphine intoxication.
AB - We developed a reliable, simple and sensitive method to determine free and total morphine in human liver and kidney, using gas chromatography-mass spectrometry (GC-MS). Free morphine or total morphine obtained by acid hydrolysis from 0.2 g tissue sample was extracted using an Extrelut® NT column with an internal standard, dihydrocodeine, followed by trimethylsilylation. The derivatized extract was submitted to GC-MS analysis of EI-SIM mode. The calibration curves of morphine in both liver and kidney samples were linear in the concentration range from 0.005 to 5 μg/g. The lower limits of detection of morphine were 0.005 μg/g. This method proved successful when we determined free and total morphine in liver and kidney obtained from an autopsied man who was mis-ingested morphine compound in the hospital, which resulted in the cause of death being morphine intoxication.
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U2 - 10.1016/j.jchromb.2005.10.049
DO - 10.1016/j.jchromb.2005.10.049
M3 - Article
C2 - 16311082
AN - SCOPUS:30444460585
SN - 1570-0232
VL - 830
SP - 359
EP - 363
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 2
ER -