Simple and easy synthesis of γ-amido-dNTPs in water and their polymerase reaction properties

Takato Sakurada, Ryo Miyahara, Ryoji Kawazoe, Yusuke Nagata, Yoshiya Kikukawa, Shigeki Sasaki, Yosuke Taniguchi

Research output: Contribution to journalArticlepeer-review

Abstract

γ-Amido-modified 2′-deoxynucleoside triphosphates (dNTPs) and nucleoside triphosphates (NTPs) are becoming increasingly important as biological tools. We herein describe the simple and easy synthesis of γ-amido-dNTPs and -NTPs from commercially available corresponding dNTPs and NTPs in a one-pot reaction using water-soluble carbodiimide and ammonia solution. We examined the effects of synthesized γ-amido-dNTPs on the DNA polymerase reaction. The results obtained showed the incorporation of these derivatives into the DNA primer while maintaining nucleobase selectivity; however, their incorporation efficiency by DNA polymerase was lower than that of dNTP. This is the first study to demonstrate the successful synthesis of four sets of γ-amido-dNTPs and clarify their properties.

Original languageEnglish
Pages (from-to)1061
Number of pages1
JournalChemical and Pharmaceutical Bulletin
Volume69
Issue number11
DOIs
Publication statusPublished - Nov 1 2021

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • Drug Discovery

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