TY - JOUR
T1 - SGV1 encodes a CDC28/cdc2-related kinase required for a Gα subunit-mediated adaptive response to pheromone in S. cerevisiae
AU - Irie, Kenji
AU - Nomoto, Satoshi
AU - Miyajima, Ikuko
AU - Matsumoto, Kunihiro
N1 - Funding Information:
We are grateful to Henry Bourne, who originally proposed the possibility that GPAl stimulates the recovery pathway. We thank Steven Reed, Jeremy Thorner, Fred Chang, Gerald Fink, Fred Cross, and Ira Her-skowitz for generously providing mutants or plasmids, or for communicating results prior to publication. We also thank Rosamaria Ruggieri and Allan Waitz for a critical reading of the manuscript. We thank DNAX Research Institute, Ken-ichi Arai, Hiroyuki Araki, Masanori Takase, and Yasuji Oshima for supporting this project. Part of this work was supported by CIBA-GEIGY Foundation (Japan) for the Prqmotion of Science.
PY - 1991/5/31
Y1 - 1991/5/31
N2 - The GPA1 gene of S. cerevisise encodes a Gα subunit that plays a positive role in the transduction of signals stimulating recovery from pheromone-induced cell cycle arrest. The GPA1Va150 mutation, in which Gly-50 is replaced by valine, causes hyperadaptation to pheromone. However, GPA1Val50 cells do not recover from division arrest in the absence of both CLN1 and CLN3, which encode G1 cyclins, indicating that the recovery-promoting activity of GPA1Val50 requires the function of G1 cyclins. An sgv1 mutation suppresses the hyperadaptive response caused by GPA1Val50 and also confers cold- and temperature-sensitive growth. The SGV1 gene encodes an apparent protein kinase homologous to CDC28/cdc2 kinase: SGV1 is 42% identical to CDC28. The activated mutation, CLN3-2, partially suppresses the growth defect of sgv1, suggesting that the SGV1 and CLN3 proteins may act in the same growth control pathway.
AB - The GPA1 gene of S. cerevisise encodes a Gα subunit that plays a positive role in the transduction of signals stimulating recovery from pheromone-induced cell cycle arrest. The GPA1Va150 mutation, in which Gly-50 is replaced by valine, causes hyperadaptation to pheromone. However, GPA1Val50 cells do not recover from division arrest in the absence of both CLN1 and CLN3, which encode G1 cyclins, indicating that the recovery-promoting activity of GPA1Val50 requires the function of G1 cyclins. An sgv1 mutation suppresses the hyperadaptive response caused by GPA1Val50 and also confers cold- and temperature-sensitive growth. The SGV1 gene encodes an apparent protein kinase homologous to CDC28/cdc2 kinase: SGV1 is 42% identical to CDC28. The activated mutation, CLN3-2, partially suppresses the growth defect of sgv1, suggesting that the SGV1 and CLN3 proteins may act in the same growth control pathway.
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U2 - 10.1016/0092-8674(91)90386-D
DO - 10.1016/0092-8674(91)90386-D
M3 - Article
C2 - 1828190
AN - SCOPUS:0025727773
SN - 0092-8674
VL - 65
SP - 785
EP - 795
JO - Cell
JF - Cell
IS - 5
ER -